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一项关于β-防御素-2和组蛋白-5作为幼儿龋齿进展诊断标志物的研究。

A study on β-defensin-2 and histatin-5 as a diagnostic marker of early childhood caries progression.

作者信息

Jurczak Anna, Kościelniak Dorota, Papież Monika, Vyhouskaya Palina, Krzyściak Wirginia

机构信息

Department of Pediatric Dentistry, Institute of Dentistry, Jagiellonian University, Medical College, Krakow, Poland.

Department of Cytobiology, Pharmacy Faculty, Jagiellonian University, Medical College, Krakow, Poland.

出版信息

Biol Res. 2015 Oct 31;48:61. doi: 10.1186/s40659-015-0050-7.

DOI:10.1186/s40659-015-0050-7
PMID:26520150
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4628373/
Abstract

BACKGROUND

Recently, a continuous growth of interest has been observed in antimicrobial peptides (AMPs) in the light of an alarming increase in resistance of bacteria and fungi against antibiotics. AMPs are used as biomarkers in diagnosis and monitoring of oral cavity pathologies. Therefore, the determination of specific protein profiles in children diagnosed with early childhood caries (ECC) might be a basis for effective screening tests and specialized examinations which may enable progression of disease.

METHODS

The objective of the studies was to determine the role of histatin-5 and β-defensing-2 as a diagnostic marker of early childhood caries progression. In this work, results of concentration determination of two salivary proteins (histatin-5 and β-defensin-2) were presented. In addition, bacterial profiles from dental plaque in various stages of ECC and control were marked. The assessment of alteration in the concentration of these two proteins in a study group of children with various stages of ECC and a control group consisting of children with no symptoms was performed by enzyme-linked immunosorbent assays.

RESULTS

The statistical analysis showed a significant increase in the concentration of histatin-5 and β-defensin-2 in the study group compared to the control group and correlated with the progression of the disease.

CONCLUSIONS

The confirmation of concentration changes in these proteins during the progression of dental caries may discover valuable disease progression biomarkers.

摘要

背景

近年来，鉴于细菌和真菌对抗生素的耐药性惊人增加，人们对抗菌肽（AMPs）的兴趣持续增长。抗菌肽被用作口腔疾病诊断和监测的生物标志物。因此，确定患有早期儿童龋（ECC）的儿童的特定蛋白质谱可能是有效筛查测试和专业检查的基础，这可能有助于疾病的进展。

方法

本研究的目的是确定组蛋白-5和β-防御素-2作为早期儿童龋进展诊断标志物的作用。在这项工作中展示了两种唾液蛋白（组蛋白-5和β-防御素-2）浓度测定的结果。此外，还标记了ECC不同阶段和对照组牙菌斑中的细菌谱。通过酶联免疫吸附测定法对患有不同阶段ECC的儿童研究组和由无症状儿童组成对照组中这两种蛋白质浓度的变化进行了评估。

结果

统计分析表明，与对照组相比，研究组中组蛋白-5和β-防御素-2的浓度显著增加，且与疾病进展相关。

结论

在龋齿进展过程中这些蛋白质浓度变化的证实可能会发现有价值疾病进展生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c2/4628373/334721395546/40659_2015_50_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c2/4628373/81ea60ca3024/40659_2015_50_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c2/4628373/ce27a113d243/40659_2015_50_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c2/4628373/334721395546/40659_2015_50_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c2/4628373/81ea60ca3024/40659_2015_50_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c2/4628373/ce27a113d243/40659_2015_50_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c2/4628373/334721395546/40659_2015_50_Fig3_HTML.jpg

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