Department of Laboratory Medicine, Gyeongsang National University Hospital, Jinju, Korea.
Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Ōmura Satoshi Memorial Institute, Kitasato University, Tokyo, Japan.
Ann Lab Med. 2022 Jul 1;42(4):438-446. doi: 10.3343/alm.2022.42.4.438.
Few studies have investigated the invasiveness of based on whole-genome sequencing (WGS). Using WGS, we determined the genomic features associated with invasiveness of strains in Korea.
Forty-five strains from 1997, 2006, and 2017, including common emm types, were selected from the repository at Gyeongsang National University Hospital in Korea. In addition, 48 strains were randomly selected depending on their invasiveness between 1997 and 2017 to evaluate the genetic evolution and the associations between invasiveness and genetic profiles. Using WGS datasets, we conducted virulence-associated DNA sequence determination, genotyping, multi-locus sequence typing (MLST), and superantigen gene profiling.
In total, 87 strains were included in this study. There were no significant differences in the genomic features throughout the study periods. Four genes, , , , and , were detected only in invasive strains. There was a significant association between invasiveness and cluster type A-C3, including, 1.0, 1.18, 1.3, and 1.76 (<0.05). The predominant 1 lineage belonged to ST28. There were no associations between invasiveness and superantigen gene profiles.
This is the first study using WGS datasets of strains collected between 1997 and 2017 in Korea. Streptococcal invasiveness is associated with the presence of , , , and . The 1 lineage and ST28 clone are explicitly associated with invasiveness, whereas genomic features remained stable over the 20-year period.
基于全基因组测序（WGS）的研究很少探讨 的侵袭性。本研究使用 WGS 确定与韩国 菌株侵袭性相关的基因组特征。
从韩国全南国立大学医院的存储库中选择了 1997 年、2006 年和 2017 年的 45 株 菌株，包括常见的 emm 型。此外，根据 1997 年至 2017 年期间的侵袭性，随机选择 48 株 菌株，以评估遗传进化以及侵袭性与遗传特征之间的关系。使用 WGS 数据集，我们进行了与毒力相关的 DNA 序列测定、 基因分型、多位点序列分型（MLST）和超抗原基因谱分析。
本研究共纳入 87 株 菌株。在整个研究期间，基因组特征没有显著差异。仅在侵袭性菌株中检测到 4 个基因， 、 、 、 。侵袭性与 簇型 A-C3，包括 、1.0、1.18、1.3 和 1.76（<0.05）之间存在显著关联。主要的 1 谱系属于 ST28。侵袭性与超抗原基因谱之间没有关联。
这是首次使用韩国 1997 年至 2017 年间收集的 菌株的 WGS 数据集进行的研究。链球菌的侵袭性与 、 、 、 的存在有关。1 谱系和 ST28 克隆与侵袭性明确相关，而基因组特征在 20 年期间保持稳定。
