Emamgholi Asgar, Rahimi Mohsen, Kaka Gholamreza, Sadraie Seyed Homayoon, Najafi Saleh
Neuroscience Research Center, Baqiyatallah University of Medical Science, Tehran, Iran ; Microbial Biotechnology, Faculty of Science, Payame Noor University, Unit of Tehran Center, Tehran, Iran.
Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Science, Tehran, Iran.
Iran J Basic Med Sci. 2015 Sep;18(9):887-93.
Various methods for repairing bone defects are presented. Cell therapy is one of these methods. Bone marrow stromal cells (BMSCs) seem to be suitable for this purpose. On the other hand, lots of biomaterials are used to improve and repair the defect in the body, so in this study we tried to produce a similar structure to the bone by the chitosan and hydroxyapatite.
In this study, the solution of chitosan-nanohydroxyapatite-polyethylene oxide (PEO) Nanofibers was produced by electrospinning method, and then the BMSCs were cultured on this solution. A piece of chitosan-nanohydroxyapatite Nanofibers with BMSCs was placed in a hole with the diameter of 1 mm at the distal epiphysis of the rat femur. Then the biomechanical and radiographic studies were performed.
Biomechanical testing results showed that bone strength was significantly higher in the Nanofiber/BMSCs group in comparison with control group. Also the bone strength in nanofiber/BMSCs group was significant, but in nanofiber group was nearly significant. Radiographic studies also showed that the average amount of callus formation (radio opacity) in nanofiber and control group was not significantly different. The callus formation in nanofiber/BMSCs group was increased compared to the control group, and it was not significant in the nanofiber group.
Since chitosan-nanohydroxyapatite nanofibers with BMSCs increases the rate of bone repair, the obtained cell-nanoscaffold shell can be used in tissue engineering and cell therapy, especially for bone defects.
介绍了多种修复骨缺损的方法。细胞治疗是其中之一。骨髓基质细胞（BMSCs）似乎适用于此目的。另一方面，许多生物材料被用于改善和修复体内缺损，因此在本研究中，我们试图用壳聚糖和羟基磷灰石制造出类似骨的结构。
在本研究中，通过静电纺丝法制备壳聚糖 - 纳米羟基磷灰石 - 聚环氧乙烷（PEO）纳米纤维溶液，然后将BMSCs接种于该溶液上。将一片含有BMSCs的壳聚糖 - 纳米羟基磷灰石纳米纤维置于大鼠股骨远端骨骺处直径为1 mm的孔中。然后进行生物力学和影像学研究。
生物力学测试结果表明，与对照组相比，纳米纤维/BMSCs组的骨强度显著更高。纳米纤维/BMSCs组的骨强度也有显著差异，但纳米纤维组几乎有显著差异。影像学研究还表明，纳米纤维组和对照组的骨痂形成平均量（放射不透性）无显著差异。纳米纤维/BMSCs组的骨痂形成较对照组增加，而纳米纤维组不显著。
由于含BMSCs的壳聚糖 - 纳米羟基磷灰石纳米纤维可提高骨修复率，所获得的细胞 - 纳米支架外壳可用于组织工程和细胞治疗，尤其是骨缺损治疗。
