Seyyed Tabaei Seyyed Javad, Rahimi Mohsen, Akbaribazm Mohsen, Ziai Seyed Ali, Sadri Minoo, Shahrokhi Seyed Reza, Rezaei Mitra Sadat
Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Parasitology and Mycology, School of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Basic Med Sci. 2020 Jun;23(6):788-799. doi: 10.22038/ijbms.2020.41361.9770.
Rapid healing of cutaneous leishmaniasis as one of the most important parasitic diseases leads to the decrease of scars and prevention of a great threat to the looks of the affected people. Today, the use of nano-scaffolds is rapidly increasing in tissue engineering and regenerative medicine with structures similar to the target tissue. Chitosan (CS) is a bioactive polymer with antimicrobial and accelerating features of healing wounds, which is commonly used in biomedicine. This study aimed to investigate the effects of CS/polyethylene oxide (PEO)/berberine (BBR) nanofibers on the experimental ulcers of in BALB/c mice.
CS/PEO/BBR nanofibers were prepared by the electrospinning method, and their morphology was examined by SEM, TEM, and AFM. Then, water absorption, stability, biocompatibility, porosity, and drug release from nano-scaffolds were explored. Afterward, 28 BALB/c mice infected with the parasite were randomly divided into control and experimental groups, and their wounds were dressed with the produced nano-scaffolds. Finally, the effect of nanobandage on the animals was investigated by macroscopic, histopathologic, and imaging examinations.
The prepared nanofibers were completely uniform, cylindrical, bead-free, and biocompatible with an average diameter of 94±12 nm and had appropriate drug release. In addition, the reduced skin ulcer diameter (0.000), parasite burden (0.003), changes in the epidermis (0.023), and dermis (0.032) indicated significantly strong effectiveness of the produced nano-scaffolds against leishmania ulcers.
Studies showed that CS/PEO/BBR nanofibers have a positive effect on the rapid healing of leishmania ulcers. Future studies should focus on other chronic ulcers treatment.
皮肤利什曼病作为最重要的寄生虫病之一，其快速愈合可减少疤痕形成，并防止对患者容貌造成巨大威胁。如今，在组织工程和再生医学中，与目标组织结构相似的纳米支架的应用正在迅速增加。壳聚糖（CS）是一种具有抗菌和促进伤口愈合功能的生物活性聚合物，常用于生物医学领域。本研究旨在探讨CS/聚环氧乙烷（PEO）/黄连素（BBR）纳米纤维对BALB/c小鼠实验性溃疡的影响。
采用静电纺丝法制备CS/PEO/BBR纳米纤维，通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）和原子力显微镜（AFM）对其形态进行观察。然后，研究纳米支架的吸水性、稳定性、生物相容性、孔隙率和药物释放情况。随后，将28只感染寄生虫的BALB/c小鼠随机分为对照组和实验组，用制备的纳米支架对其伤口进行包扎。最后，通过宏观、组织病理学和影像学检查研究纳米绷带对动物的影响。
制备的纳米纤维完全均匀、呈圆柱形、无珠粒，具有生物相容性，平均直径为94±12 nm，且具有适当的药物释放性能。此外，皮肤溃疡直径减小（0.000）、寄生虫负荷降低（0.003）、表皮变化（0.023）和真皮变化（0.032）表明，所制备的纳米支架对利什曼溃疡具有显著的强效作用。
研究表明，CS/PEO/BBR纳米纤维对利什曼溃疡的快速愈合具有积极作用。未来的研究应聚焦于其他慢性溃疡的治疗。
