Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology , 38106 Braunschweig, Germany.
Nano Lett. 2015 Dec 9;15(12):8354-9. doi: 10.1021/acs.nanolett.5b04045. Epub 2015 Nov 5.
Optical nanoantennas are known to focus freely propagating light and reversely to mediate the emission of a light source located at the nanoantenna hotspot. These effects were previously exploited for fluorescence enhancement and single-molecule detection at elevated concentrations. We present a new generation of self-assembled DNA origami based optical nanoantennas with improved robustness, reduced interparticle distance, and optimized quantum-yield improvement to achieve more than 5000-fold fluorescence enhancement and single-molecule detection at 25 μM background fluorophore concentration. Besides outperforming lithographic optical antennas, DNA origami nanoantennas are additionally capable of incorporating single emitters or biomolecular assays at the antenna hotspot.
光学纳米天线可以聚焦自由传播的光，并反向介导位于纳米天线热点处光源的发射。这些效应之前被用于在高浓度下增强荧光和检测单分子。我们提出了一种新一代基于自组装 DNA 折纸的光学纳米天线，具有更好的稳定性、更小的颗粒间距离和优化的量子产率提高，可在 25 μM 背景荧光团浓度下实现超过 5000 倍的荧光增强和单分子检测。除了性能优于光刻光学天线外，DNA 折纸纳米天线还可以在天线热点处结合单发射体或生物分子检测。
