Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology , 38106 Braunschweig, Germany.
Institute of Physics, Faculty of Physics, Astronomy, and Informatics, Nicolaus Copernicus University , Grudziadzka 5, 87-100 Torun, Poland.
ACS Nano. 2018 Feb 27;12(2):1650-1655. doi: 10.1021/acsnano.7b08233. Epub 2018 Jan 29.
In this contribution, we fabricate hybrid constructs based on a natural light-harvesting complex, peridinin-chlorophyll a-protein, coupled to dimer optical antennas self-assembled with the help of the DNA origami technique. This approach enables controlled positioning of individual complexes at the hotspot of the optical antennas based on large, colloidal gold and silver nanoparticles. Our approach allows us to selectively excite the different pigments present in the harvesting complex, reaching a fluorescence enhancement of 500-fold. This work expands the range of self-assembled functional hybrid constructs for harvesting sunlight and can be further developed for other pigment-proteins and proteins.
在本研究中，我们采用一种自然的光捕获复合物（别藻蓝蛋白），将其与利用 DNA 折纸技术自组装的二聚体光天线相偶联，从而构建出混合结构。该方法利用大尺寸胶体金和银纳米粒子，实现了基于单个复合物在光天线热点处的可控定位。通过这种方法，我们可以选择性地激发光捕获复合物中存在的不同颜料，从而实现 500 倍的荧光增强。这项工作扩展了用于收集太阳光的自组装功能混合结构的范围，并且可以进一步应用于其他色素蛋白和蛋白。
