Adesina Simeon K, Akala Emmanuel O
Department of Pharmaceutical Sciences, Howard University , Washington, DC 20059, United States.
Mol Pharm. 2015 Dec 7;12(12):4175-87. doi: 10.1021/acs.molpharmaceut.5b00335. Epub 2015 Nov 9.
RNA interference (RNAi) is triggered by oligonucleotides that are about 21-23 nucleotides long and are capable of inducing the destruction of complementary mRNA. The RNAi technique has been successfully utilized to target HIV replication; however, the main limitation to the successful utilization of this technique in vivo is the inability of naked siRNA to cross the cell membrane by diffusion due to its strong anionic charge and large molecular weight. This review describes current nonviral nanotechnological approaches to deliver anti-HIV siRNAs for the treatment of HIV infection.
RNA干扰（RNAi）由长度约为21 - 23个核苷酸的寡核苷酸引发，这些寡核苷酸能够诱导互补mRNA的降解。RNAi技术已成功用于靶向HIV复制；然而，该技术在体内成功应用的主要限制是，由于其强阴离子电荷和大分子量，裸露的小干扰RNA（siRNA）无法通过扩散穿过细胞膜。本综述描述了目前用于递送抗HIV siRNA以治疗HIV感染的非病毒纳米技术方法。
