Department of Materials Science and Engineering, The Ohio State University, Columbus, OH, USA.
Department of Biomedical Engineering, The Ohio State University, Columbus, OH, USA.
Biomaterials. 2016 Jan;76:208-17. doi: 10.1016/j.biomaterials.2015.10.055. Epub 2015 Oct 23.
To fully understand biological behavior in vitro often dictates that oxygen be reported at either a local or a cellular level. Oxygen sensors based on the luminescent quenching of a specific form of electrospun fiber were developed for measurement of both gaseous and dissolved oxygen concentrations. Electrospinning was used to fabricate "core-shell" fiber configurations in which oxygen-sensitive transition-metal porphyrin complexes are embedded in an optically clear, gas permeable polycarbonate polymer 'core' while polycaprolactone provided a protective yet biocompatible 'shell'. By taking advantage of the resulting high sensitivity and fast response of electrospun core-shell fiber sensors, we were able to locate and image hypoxic regions in contact with aggregates of glioblastoma cells. Nanoscale, biomimetic sensors containing oxygen-sensitive porphyrins are particularly well suited to biological applications. These 'smart' nanofiber based sensors do not consume oxygen, their mechanical and chemical characteristics can be finely tuned allowing tailoring of biocompatibility and microstructure. Core-shell nanofiber oxygen sensing fibers could provide real-time assessments of tumor cell response to pharmacological innovations designed to target hypoxic regions driving new knowledge and technological advancement.
要充分了解体外的生物行为，通常需要在局部或细胞水平上报告氧含量。为此，开发了基于特定形式的电纺纤维的发光猝灭的氧传感器，用于测量气态和溶解氧浓度。电纺用于制造“核壳”纤维结构，其中氧敏过渡金属卟啉配合物嵌入在光学透明、透气的聚碳酸酯聚合物“核”中，而聚己内酯则提供了保护性且生物相容的“壳”。利用电纺核壳纤维传感器的高灵敏度和快速响应的优势，我们能够定位和成像与神经胶质瘤细胞聚集物接触的缺氧区域。含有氧敏卟啉的纳米级仿生传感器特别适合于生物应用。这些“智能”基于纳米纤维的传感器不消耗氧气，其机械和化学特性可以精细调整，从而实现生物相容性和微观结构的定制。核壳纳米纤维氧传感纤维可以实时评估肿瘤细胞对旨在靶向驱动新知识和技术进步的缺氧区域的药理创新的反应。
