Ferreira Fernando Costa, Soares Maria João, Carvalho Sandra, Borralho Liliana, Vicente Gonçalo, Branco Sandra, Correia Jorge, Peleteiro Maria Conceição
CIISA, Interdisciplinary Centre of Research in Animal Health, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477, Lisbon, Portugal.
Clinica Veterinária do Oriente, Rua Nova dos Mercadores, 13 R/C Dto. 1990-176, Lisboa, Portugal.
Diagn Pathol. 2015 Nov 2;10:199. doi: 10.1186/s13000-015-0429-3.
Four cases of tumors in which cell internalization was frequently visualized are reported: one feline mammary carcinoma, one feline cutaneous squamous cell carcinoma, one canine pulmonary squamous cell carcinoma and one canine pleural mesothelioma. Cell internalization was observed by cytology in two of these cases (the feline mammary tumour and the pleural effusion in the canine mesothelioma) and by histopathology in all but the canine mesothelioma. Immunohistochemical staining for pancytokeratin was positive for both internalized and host cells, while E-cadherin expression was frequently absent, although internalized cells occasionally stained positive. This cell-to-cell interaction seems to be associated with tumors displaying a strong epithelial-mesenchymal transitional phenotype, in which cancer cells become engulfed by other cancer cells. Such event could be regarded as an important hallmark of very high malignancy.
报告了4例经常可见细胞内化的肿瘤病例：1例猫乳腺腺癌、1例猫皮肤鳞状细胞癌、1例犬肺鳞状细胞癌和1例犬胸膜间皮瘤。其中2例（猫乳腺肿瘤和犬间皮瘤的胸腔积液）通过细胞学观察到细胞内化，除犬间皮瘤外，其他病例均通过组织病理学观察到细胞内化。全细胞角蛋白免疫组化染色显示内化细胞和宿主细胞均呈阳性，而E-钙黏蛋白表达通常缺失，尽管内化细胞偶尔呈阳性染色。这种细胞间相互作用似乎与表现出强烈上皮-间质转化表型的肿瘤有关，其中癌细胞被其他癌细胞吞噬。这种事件可被视为极高恶性肿瘤的一个重要标志。
