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G2-EPSPS和草甘膦乙酰转移酶GAT基因共表达赋予大豆对草甘膦的高耐受性。

Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean.

作者信息

Guo Bingfu, Guo Yong, Hong Huilong, Jin Longguo, Zhang Lijuan, Chang Ru-Zhen, Lu Wei, Lin Min, Qiu Li-Juan

机构信息

The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences , Beijing, China ; College of Agriculture, Northeast Agricultural University , Harbin, China.

The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences , Beijing, China.

出版信息

Front Plant Sci. 2015 Oct 15;6:847. doi: 10.3389/fpls.2015.00847. eCollection 2015.

DOI:10.3389/fpls.2015.00847
PMID:26528311
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4606067/
Abstract

Glyphosate is a widely used non-selective herbicide with broad spectrum of weed control around the world. At present, most of the commercial glyphosate tolerant soybeans utilize glyphosate tolerant gene CP4-EPSPS or glyphosate acetyltransferase gene GAT separately. In this study, both glyphosate tolerant gene G2-EPSPS and glyphosate degraded gene GAT were co-transferred into soybean and transgenic plants showed high tolerance to glyphosate. Molecular analysis including PCR, Sothern blot, qRT-PCR, and Western blot revealed that target genes have been integrated into genome and expressed effectively at both mRNA and protein levels. Furthermore, the glyphosate tolerance analysis showed that no typical symptom was observed when compared with a glyphosate tolerant line HJ06-698 derived from GR1 transgenic soybean even at fourfold labeled rate of Roundup. Chlorophyll and shikimic acid content analysis of transgenic plant also revealed that these two indexes were not significantly altered after glyphosate application. These results indicated that co-expression of G2-EPSPS and GAT conferred high tolerance to the herbicide glyphosate in soybean. Therefore, combination of tolerant and degraded genes provides a new strategy for developing glyphosate tolerant transgenic crops.

摘要

草甘膦是一种广泛使用的非选择性除草剂，在全球范围内具有广泛的杂草防治谱。目前，大多数商业化的耐草甘膦大豆分别利用耐草甘膦基因CP4-EPSPS或草甘膦乙酰转移酶基因GAT。在本研究中，耐草甘膦基因G2-EPSPS和草甘膦降解基因GAT被共同转入大豆，转基因植株对草甘膦表现出高耐受性。包括PCR、Southern杂交、qRT-PCR和Western杂交在内的分子分析表明，目标基因已整合到基因组中，并在mRNA和蛋白质水平上有效表达。此外，草甘膦耐受性分析表明，即使在农达四倍标记剂量下，与源自GR1转基因大豆的耐草甘膦品系HJ06-698相比，也未观察到典型症状。转基因植株的叶绿素和莽草酸含量分析还表明，施用草甘膦后这两个指标没有显著变化。这些结果表明，G2-EPSPS和GAT的共表达赋予了大豆对除草剂草甘膦的高耐受性。因此，耐受基因和降解基因的组合为培育耐草甘膦转基因作物提供了一种新策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/ed2d67f8ba4e/fpls-06-00847-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/fb1a47d35185/fpls-06-00847-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/2cea47bb5ccf/fpls-06-00847-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/745a158436b8/fpls-06-00847-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/65b068d86983/fpls-06-00847-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/140a13d22f0f/fpls-06-00847-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/ed2d67f8ba4e/fpls-06-00847-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/fb1a47d35185/fpls-06-00847-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/2cea47bb5ccf/fpls-06-00847-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/745a158436b8/fpls-06-00847-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/65b068d86983/fpls-06-00847-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/140a13d22f0f/fpls-06-00847-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a5/4606067/ed2d67f8ba4e/fpls-06-00847-g0006.jpg

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