核糖体RNA操纵子位于小质粒而非染色体上的细菌进化枝。
Bacterial clade with the ribosomal RNA operon on a small plasmid rather than the chromosome.
作者信息
Anda Mizue, Ohtsubo Yoshiyuki, Okubo Takashi, Sugawara Masayuki, Nagata Yuji, Tsuda Masataka, Minamisawa Kiwamu, Mitsui Hisayuki
机构信息
Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577, Japan.
Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577, Japan
出版信息
Proc Natl Acad Sci U S A. 2015 Nov 17;112(46):14343-7. doi: 10.1073/pnas.1514326112. Epub 2015 Nov 3.
rRNA is essential for life because of its functional importance in protein synthesis. The rRNA (rrn) operon encoding 16S, 23S, and 5S rRNAs is located on the "main" chromosome in all bacteria documented to date and is frequently used as a marker of chromosomes. Here, our genome analysis of a plant-associated alphaproteobacterium, Aureimonas sp. AU20, indicates that this strain has its sole rrn operon on a small (9.4 kb), high-copy-number replicon. We designated this unusual replicon carrying the rrn operon on the background of an rrn-lacking chromosome (RLC) as the rrn-plasmid. Four of 12 strains close to AU20 also had this RLC/rrn-plasmid organization. Phylogenetic analysis showed that those strains having the RLC/rrn-plasmid organization represented one clade within the genus Aureimonas. Our finding introduces a previously unaddressed viewpoint into studies of genetics, genomics, and evolution in microbiology and biology in general.
由于rRNA在蛋白质合成中具有重要功能，所以它对生命至关重要。编码16S、23S和5S rRNA的rRNA（rrn）操纵子位于所有迄今已记录的细菌的“主”染色体上，并且经常被用作染色体的标记。在此，我们对一种与植物相关的α-变形菌Aureimonas sp. AU20进行了基因组分析，结果表明该菌株在一个小的（9.4 kb）、高拷贝数的复制子上有其唯一的rrn操纵子。我们将这种在缺乏rrn的染色体（RLC）背景下携带rrn操纵子的不寻常复制子命名为rrn质粒。与AU20亲缘关系较近的12个菌株中有4个也具有这种RLC/rrn质粒结构。系统发育分析表明，那些具有RLC/rrn质粒结构的菌株在Aureimonas属内代表一个分支。我们的发现为微生物学及整个生物学领域的遗传学、基因组学和进化研究引入了一个此前未涉及的观点。
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