Park Jisu, Chung Heesung, Bang Seung Hyun, Han Ah-Reum, Seo Eun-Kyoung, Chang Sung Eun, Kang Duk-Hee, Oh Eok-Soo
Department of Life Sciences, the Research Center for Cellular Homeostasis, Ewha Womans University, Seoul, Korea.
Department of Dermatology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
PLoS One. 2015 Nov 4;10(11):e0141988. doi: 10.1371/journal.pone.0141988. eCollection 2015.
We investigated the potential melanogenic effect of compounds from Zingiber cassumunar Roxb. Our data revealed that chloroform-soluble extract of Z. cassumunar enhanced melanin synthesis in B16F10 melanoma cells. Among the components of the chloroform extract, (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-ol (DMPB) increased melanogenesis in both B16F10 cells and human primary melanocytes. In B16F10 cells, DMPB enhanced the activation of ERK and p38, and the level of tyrosinase. Although the level of microphthalmia-associated transcription factor was unchanged in DMPB-treated B16F10 cells, DMPB increased levels and nuclear localization of upstream stimulating factor-1 (USF1). Consistently, DMPB-mediated melanin synthesis was diminished in USF1-knockdown cells. Furthermore, DMPB induced hyperpigmentation in brown guinea pigs in vivo. Together, these data suggest that DMPB may promote melanin synthesis via USF1 dependent fashion and could be used as a clinical therapeutic agent against hypopigmentation-associated diseases.
我们研究了高良姜化合物的潜在黑素生成作用。我们的数据显示，高良姜的氯仿可溶提取物增强了B16F10黑色素瘤细胞中的黑色素合成。在氯仿提取物的成分中，（E）-4-（3,4-二甲氧基苯基）丁-3-烯-1-醇（DMPB）在B16F10细胞和人原代黑素细胞中均增加了黑色素生成。在B16F10细胞中，DMPB增强了ERK和p38的激活以及酪氨酸酶的水平。尽管在DMPB处理的B16F10细胞中小眼畸形相关转录因子的水平没有变化，但DMPB增加了上游刺激因子-1（USF1）的水平和核定位。一致地，在USF1敲低的细胞中，DMPB介导的黑色素合成减少。此外，DMPB在体内诱导棕色豚鼠色素沉着过度。总之，这些数据表明DMPB可能通过USF1依赖性方式促进黑色素合成，并可作为治疗色素减退相关疾病的临床治疗剂。
