Liu Lifeng, Zhang Jinliang, Lu Yan, Fang Chunfang, Li Senlin, Lin Jusheng
Department of Gastroenterology, Liaocheng People's Hospital, Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong, China.
Department of Hepatobiliary Surgery, Liaocheng People's Hospital, Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong, China.
PLoS One. 2015 Nov 4;10(11):e0141861. doi: 10.1371/journal.pone.0141861. eCollection 2015.
We have previously identified 8 SNPs in Han Chinese HBV carriers that are associated with disease progression. Although not well studied, genetic factors may also play a significant role in developing chronic HBV disease after exposure. We extend the effect of these eight SNPs on persistent HBV infection in this study. A total of 875 unrelated Han Chinese, 493 chronic hepatitis B subjects (CHB) and 382 HBV clearance individuals (Clear), were recruited from Hubei Province from September 2007 to March 2010. SNPs were verified by using TaqMan 7900HT Sequence Detection System. By using multiple logistic regression analysis, each of the 8 SNP associations was tested using 3 different genetic models (Dominant, Recessive and Additive model), in 4 types of analyses (full sample, men, women, age stratified). A Bonferroni correction was used to account for multiple statistical tests for each SNP association (P<0.05/8 = 0.0063). A significant correlation was observed at SNP rs10485138 located in ASCC3 gene in female patients (OR, 0.445; 95% CI, 0.253-0.784; P = 0.005). Females bearing C allele infected by HBV had an increased susceptibility to CHB compared with those T allele carriers. Our results indicated that SNP rs10485138 located in ASCC3 gene was associated with persistent HBV infection in Han Chinese.
我们之前在汉族乙肝病毒携带者中鉴定出8个与疾病进展相关的单核苷酸多态性（SNP）。尽管研究尚不充分，但遗传因素在接触乙肝病毒后慢性乙肝疾病的发生中可能也起着重要作用。在本研究中，我们扩展了这8个SNP对持续性乙肝病毒感染的影响。2007年9月至2010年3月期间，从湖北省招募了总共875名无亲缘关系的汉族人，其中493名慢性乙型肝炎患者（CHB）和382名乙肝病毒清除者（Clear）。使用TaqMan 7900HT序列检测系统对SNP进行验证。通过多因素逻辑回归分析，在4种分析类型（全样本、男性、女性、年龄分层）中，使用3种不同的遗传模型（显性、隐性和加性模型）对8个SNP关联中的每一个进行检验。采用Bonferroni校正来处理每个SNP关联的多重统计检验（P<0.05/8 = 0.0063）。在女性患者中，位于ASCC3基因的SNP rs10485138观察到显著相关性（比值比，0.445；95%可信区间，0.253 - 0.784；P = 0.005）。与携带T等位基因者相比，携带C等位基因的感染乙肝病毒的女性患慢性乙型肝炎的易感性增加。我们的结果表明，位于ASCC3基因的SNP rs10485138与汉族人群的持续性乙肝病毒感染相关。
