Yang Sung-Hyun, Seo Hyun-Seok, Lee Jung-Hyun, Kim Sang-Jin, Kyoung Kwon Kae
Marine Biotechnology Research Center, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Jan;66(1):430-434. doi: 10.1099/ijsem.0.000734. Epub 2015 Nov 3.
A Gram-stain-negative, aerobic, rod-shaped and non-motile marine bacterium, designated MEBiC08749T, was isolated from a marine conch collected at Samcheok Port in the East Sea, Korea (also known as the Sea of Japan). 16S rRNA gene sequence analysis revealed that strain MEBiC08749T shared highest similarity with Pseudofulvibacter geojedonensis YCS-9T (94.9 %). Growth was observed at 5-31 °C (optimum, 25 °C), at pH 6.0-8.5 (optimum, pH 7.5) and with 0-6 % (w/v) NaCl (optimum, 2.5 %). The predominant cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 9 (comprising 10-methyl C16 : 0 and/or iso-C17 : 1ω9c). The DNA G+C content was 30 mol%. The only respiratory quinone was menaquinone-6 (MK-6). Phosphatidylethanolamine, two unidentified lipids and two unidentified aminolipids were detected as major polar lipids. Several phenotypic characteristics, such as production of acetoin, activity of cystine arylamidase, utilization of some carbohydrates, organic acids and amino acids, differentiated strain MEBiC08749T from P. geojedonensis KCTC 23884T. On the basis of data from this polyphasic taxonomic study, strain MEBiC08749T should be classified as representing a novel species in the genus Pseudofulvibacter, for which the name Pseudofulvibacter gastropodicola sp. nov. is proposed. The type strain is MEBiC08749T ( = KCCM 43046T = JCM 30369T). Emended descriptions of the genus PseudofulvibacterYoon et al. 2013 and of Pseudofulvibacter geojedonensis are also given.
从韩国东海三陟港（又称日本海）采集的海洋海螺中分离出一株革兰氏染色阴性、需氧、杆状且无运动性的海洋细菌，命名为MEBiC08749T。16S rRNA基因序列分析表明，菌株MEBiC08749T与格氏假黄杆菌YCS-9T的相似度最高（94.9%）。在5-31°C（最适温度25°C）、pH 6.0-8.5（最适pH 7.5）和0-6%（w/v）NaCl（最适浓度2.5%）条件下可观察到生长。主要的细胞脂肪酸为异-C15:0、异-C15:1 G、异-C15:0 3-OH、异-C17:0 3-OH、特征性总和3（包含C16:1ω6c和/或C16:1ω7c）和特征性总和9（包含10-甲基C16:0和/或异-C17:1ω9c）。DNA G+C含量为30 mol%。唯一的呼吸醌为甲基萘醌-6（MK-6）。检测到磷脂酰乙醇胺、两种未鉴定的脂质和两种未鉴定的氨基脂质为主要极性脂质。一些表型特征，如乙酰甲基甲醇的产生、胱氨酸芳基酰胺酶的活性、对某些碳水化合物、有机酸和氨基酸的利用，使菌株MEBiC08749T与格氏假黄杆菌KCTC 23884T区分开来。基于这一多相分类研究的数据，菌株MEBiC08749T应被归类为假黄杆菌属中的一个新物种，为此提出名称为嗜螺假黄杆菌（Pseudofulvibacter gastropodicola sp. nov.）。模式菌株为MEBiC08749T（=KCCM 43046T =JCM 30369T）。还给出了假黄杆菌属（Yoon等人，2013年）和格氏假黄杆菌的修订描述。
