Kang Qunfu, Liu Weihong, Liu Hongxu, Zhou Mingxue
Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China.
Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China ; Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China.
Evid Based Complement Alternat Med. 2015;2015:584596. doi: 10.1155/2015/584596. Epub 2015 Oct 11.
Compound Chuanxiong Capsule (CCC), a Chinese herbal compound, can exhibit antiatherosclerotic effect; however, its mechanism is still unclear. This study is designed to study the mechanism of CCC on atherosclerosis in the ApoE-knockout (ApoE(-/-)) mice fed with a high-fat diet. After 6 weeks of high-fat feeding, 40 ApoE(-/-) mice were randomized (n = 10) and treated with lipitor, high-dose or low-dose CCC, or distilled water (ApoE(-/-) group) for 7 weeks. The blood lipids in serum and the plaque areas of the mice were measured and the mRNA expressions of phosphatidylinositol-3-kinases (PI3K), Akt, nuclear factor-kappa B (NF-κB), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) of the aortae were determined. The data showed that CCC can significantly decrease the levels of blood lipids, atherosclerosis index, and plaque areas and increase collagen proportion in plaques as compared with the untreated mice (p < 0.05, p < 0.01). In addition, CCC can significantly reduce the mRNA expressions of PI3K, Akt, NF-κB, IL-6, and TNF-α in the mice fed with a high-fat diet (p < 0.001). Thus, we concluded that CCC can inhibit inflammatory reaction in the ApoE(-/-) mice fed with a high-fat diet. This mechanism may be attributed to regulating PI3K/Akt/NF-κB signaling pathway.
复方川芎胶囊（CCC）是一种中药复方制剂，具有抗动脉粥样硬化作用，但其作用机制尚不清楚。本研究旨在探讨CCC对高脂喂养的载脂蛋白E基因敲除（ApoE(-/-)）小鼠动脉粥样硬化的作用机制。高脂喂养6周后，将40只ApoE(-/-)小鼠随机分为4组（每组10只），分别给予立普妥、高剂量或低剂量CCC或蒸馏水（ApoE(-/-)组）处理7周。检测小鼠血清血脂水平和斑块面积，并测定主动脉中磷脂酰肌醇-3激酶（PI3K）、蛋白激酶B（Akt）、核因子-κB（NF-κB）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的mRNA表达。结果显示，与未处理小鼠相比，CCC可显著降低血脂水平、动脉粥样硬化指数和斑块面积，并增加斑块中胶原比例（p < 0.05，p < 0.01）。此外，CCC可显著降低高脂喂养小鼠PI3K、Akt、NF-κB、IL-6和TNF-α的mRNA表达（p < 0.001）。因此，我们得出结论，CCC可抑制高脂喂养的ApoE(-/-)小鼠的炎症反应。其机制可能与调节PI3K/Akt/NF-κB信号通路有关。
