Mazzeo Maria Fiorella, Lippolis Rosa, Sorrentino Alida, Liberti Sarah, Fragnito Federica, Siciliano Rosa Anna
Institute of Food Sciences, National Research Council, Avellino, Italy.
Institute of Biomembranes and Bioenergetics, National Research Council, Bari, Italy.
PLoS One. 2015 Nov 6;10(11):e0142376. doi: 10.1371/journal.pone.0142376. eCollection 2015.
Dietary polyphenols are bioactive molecules that beneficially affect human health, due to their anti-oxidant, anti-inflammatory, cardio-protective and chemopreventive properties. They are absorbed in a very low percentage in the small intestine and reach intact the colon, where they are metabolized by the gut microbiota. Although it is well documented a key role of microbial metabolism in the absorption of polyphenols and modulation of their biological activity, molecular mechanisms at the basis of the bacteria-polyphenols interplay are still poorly understood. In this context, differential proteomics was applied to reveal adaptive response mechanisms that enabled a potential probiotic Lactobacillus acidophilus strain to survive in the presence of the dietary polyphenol rutin. The response to rutin mainly modulated the expression level of proteins involved in general stress response mechanisms and, in particular, induced the activation of protein quality control systems, and affected carbohydrate and amino acid metabolism, protein synthesis and cell wall integrity. Moreover, rutin triggered the expression of proteins involved in oxidation-reduction processes.This study provides a first general view of the impact of dietary polyphenols on metabolic and biological processes of L. acidophilus.
膳食多酚是一类生物活性分子，因其具有抗氧化、抗炎、心脏保护和化学预防特性，对人体健康有益。它们在小肠中的吸收率极低，并完整地到达结肠，在那里被肠道微生物群代谢。尽管微生物代谢在多酚吸收及其生物活性调节中起关键作用已得到充分证明，但细菌与多酚相互作用的分子机制仍知之甚少。在此背景下，采用差异蛋白质组学来揭示潜在的益生菌嗜酸乳杆菌菌株在膳食多酚芦丁存在下存活的适应性反应机制。对芦丁的反应主要调节了参与一般应激反应机制的蛋白质的表达水平，特别是诱导了蛋白质质量控制系统的激活，并影响了碳水化合物和氨基酸代谢、蛋白质合成以及细胞壁完整性。此外，芦丁触发了参与氧化还原过程的蛋白质的表达。本研究首次全面阐述了膳食多酚对嗜酸乳杆菌代谢和生物学过程的影响。
