Khan Wahid Ali, Qureshi Javed Anwer
Department of Clinical Biochemistry, College of Medicine, King Khalid University, Abha, Saudi Arabia.
APMIS. 2015 Dec;123(12):1016-24. doi: 10.1111/apm.12464.
Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease characterized by various types of immunological abnormalities including circulating and tissue-fixed autoantibodies reactive with autoantigens. The mechanism that can explain the production of these antibodies is unclear. Here we address the binding specificity of SLE autoantibodies with recombinant alpha interferon 2b (hrIFN α-2b), commercially available interferon (IFN α-2b), and the gene (cIFN α-2b) encoding this interferon. hrIFN α-2b showed higher binding with naturally occurring SLE autoantibodies as compared to IFN α-2b (p < 0.05) or cIFN α-2b gene (p < 0.001) as assessed by direct binding, inhibition ELISA, and quantitative precipitin titration. The relative affinity of SLE autoantibodies for hrIFN α-2b, IFN α-2b, and cIFN α-2b gene was in the order of 1.13 × 10(-7) , 1.38 × 10(-6) , and 1.22 × 10(-6) , respectively. hrIFN α-2b is shown to have unique epitopes that would explain the possible antigenic role of hrIFN α-2b in the generation of SLE autoantibodies. Anti-hrIFN α-2b antibodies have been shown to represent an alternative immunological probe for the estimation of interferon alpha 2b in the serum of SLE patients.
系统性红斑狼疮（SLE）是一种多系统自身免疫性疾病，其特征为多种类型的免疫异常，包括与自身抗原反应的循环性和组织固定性自身抗体。能够解释这些抗体产生的机制尚不清楚。在此，我们探讨了SLE自身抗体与重组α干扰素2b（hrIFNα - 2b）、市售干扰素（IFNα - 2b）以及编码该干扰素的基因（cIFNα - 2b）的结合特异性。通过直接结合、抑制ELISA和定量沉淀素滴定评估，与IFNα - 2b（p < 0.05）或cIFNα - 2b基因（p < 0.001）相比，hrIFNα - 2b与天然存在的SLE自身抗体表现出更高的结合力。SLE自身抗体对hrIFNα - 2b、IFNα - 2b和cIFNα - 2b基因的相对亲和力分别为1.13×10⁻⁷、1.38×10⁻⁶和1.22×10⁻⁶。hrIFNα - 2b显示具有独特的表位，这可以解释hrIFNα - 2b在SLE自身抗体产生中可能的抗原作用。抗hrIFNα - 2b抗体已被证明是用于估计SLE患者血清中α干扰素2b的另一种免疫探针。
