Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia , Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy.
The Biorobotics Institute, Scuola Superiore Sant'Anna , Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy.
ACS Appl Mater Interfaces. 2015 Nov 25;7(46):25574-9. doi: 10.1021/acsami.5b08764. Epub 2015 Nov 10.
In this letter, we report on the fabrication, the characterization, and the in vitro testing of structures suitable for cell culturing, prepared through two-photon polymerization of a nanocomposite resist. More in details, commercially available Ormocomp has been doped with piezoelectric barium titanate nanoparticles, and bioinspired 3D structures resembling trabeculae of sponge bone have been fabricated. After an extensive characterization, preliminary in vitro testing demonstrated that both the topographical and the piezoelectric cues of these scaffolds are able to enhance the differentiation process of human SaOS-2 cells.
在这封信中，我们报告了通过双光子聚合纳米复合光刻胶制备适用于细胞培养的结构的制造、表征和体外测试。更详细地说，我们在市售的 Ormocomp 中掺杂了压电钛酸钡纳米颗粒，并制备了类似海绵骨小梁的仿生 3D 结构。经过广泛的表征，初步的体外测试表明，这些支架的形貌和压电线索都能够增强人 SaOS-2 细胞的分化过程。
