Shi Jue, Liu Yu, Wang Ying, Zhang Jing, Zhao Shifang, Yang Guoli
Department of Oral and Maxillofacial Surgery, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
Department of Oral Medicine, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
Sci Rep. 2015 Nov 9;5:16336. doi: 10.1038/srep16336.
The prevention and control of peri-implantitis is a challenge in dental implant surgery. Dental implants with sustained antimicrobial coating are an ideal way of preventing peri-implantitis. This study reports development of a non- immunotoxicity multilayered coating on a titanium surface that had sustained antimicrobial activity and limited early biofilm formation. In this study, the broad spectrum AMP, Tet213, was linked to collagen IV through sulfo-SMPB and has been renamed as AMPCol. The multilayer AMPCol coatings were assembled on smooth titanium surfaces using a LBL technique. Using XPS, AFM, contact angle analysis, and QCM, layer-by-layer accumulation of coating thickness was measured and increased surface wetting compared to controls was confirmed. Non-cytotoxicity to HaCaT and low erythrocyte hemolysis by the AMPCol coatings was observed. In vivo immunotoxicity assays showed IP administration of AMPCol did not effect serum immunoglobulin levels. This coating with controlled release of AMP decreased the growth of both a Gram-positive aerobe (Staphylococcus aureus) and a Gram-negative anaerobe (Porphyromonas gingivalis) up to one month. Early S. aureus biofilm formation was inhibited by the coating. The excellent long-term sustained antimicrobial activity of this multilayer coating is a potential method for preventing peri-implantitis through coated on the neck of implants before surgery.
种植体周围炎的防治是牙种植手术中的一项挑战。具有持续抗菌涂层的牙种植体是预防种植体周围炎的理想方式。本研究报告了在钛表面开发一种具有持续抗菌活性且能限制早期生物膜形成的非免疫毒性多层涂层。在本研究中，广谱抗菌肽Tet213通过磺基-SMPB与IV型胶原相连，并重新命名为AMPCol。采用层层自组装技术将多层AMPCol涂层组装在光滑的钛表面。通过X射线光电子能谱（XPS）、原子力显微镜（AFM）、接触角分析和石英晶体微天平（QCM），测量了涂层厚度的逐层累积情况，并证实与对照组相比表面润湿性增加。观察到AMPCol涂层对HaCaT细胞无细胞毒性且对红细胞溶血率低。体内免疫毒性试验表明，腹腔注射AMPCol不影响血清免疫球蛋白水平。这种具有抗菌肽控释功能的涂层在长达一个月的时间内降低了革兰氏阳性需氧菌（金黄色葡萄球菌）和革兰氏阴性厌氧菌（牙龈卟啉单胞菌）的生长。该涂层抑制了金黄色葡萄球菌早期生物膜的形成。这种多层涂层优异的长期持续抗菌活性是一种在手术前通过在种植体颈部涂层来预防种植体周围炎的潜在方法。
