Neves Mônica A S, Provenzano José C, Rôças Isabela N, Siqueira José F
Department of Endodontics and Molecular Microbiology Laboratory, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Rio de Janeiro, Brazil.
Department of Endodontics and Molecular Microbiology Laboratory, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Rio de Janeiro, Brazil.
J Endod. 2016 Jan;42(1):25-9. doi: 10.1016/j.joen.2015.09.019. Epub 2015 Nov 6.
This in vivo study compared the antibacterial effectiveness of a reciprocating single-instrument system (Reciproc; VDW, Munich, Germany) and a rotary multi-instrument system (BioRaCe; FKG Dentaire, La Chaux-de-Fonds, Switzerland) during the preparation of infected root canals of teeth with primary apical periodontitis.
Root canals from single-rooted teeth with necrotic pulps and apical periodontitis were instrumented using either Reciproc (n = 29) or BioRaCe (n = 30) instruments under irrigation with 2.5% sodium hypochlorite. DNA was extracted from samples taken before and after preparation and subjected to quantitative analysis of total bacteria and streptococci by using the quantitative real-time polymerase chain reaction.
All initial samples were positive for the presence of bacteria, with median numbers of 7.1 × 10(5) and 1.31 × 10(5) bacterial cells for the Reciproc and BioRaCe groups, respectively. After preparation with Reciproc and BioRaCe, 16 (55%) and 15 (50%) root canals still had detectable bacteria with median counts of 7.05 × 10(2) and 6.03 × 10(1), respectively. Both systems were highly effective in reducing the total bacterial counts (P < .001), and there were no significant differences between them (P > .05). Streptococci were highly frequent, and both systems succeeded in significantly reducing their levels (P < .001).
Both reciprocating single-instrument and rotary multi-instrument systems were highly effective in reducing the counts of total bacteria and streptococci in root canals of teeth with apical periodontitis. Regardless of the system used, approximately one half of the teeth still had detectable bacteria.
本体内研究比较了往复式单器械系统（Reciproc；德国慕尼黑VDW公司）和旋转多器械系统（BioRaCe；瑞士拉绍德封FKG Dentaire公司）在预备患有原发性根尖周炎牙齿的感染根管时的抗菌效果。
使用Reciproc（n = 29）或BioRaCe（n = 30）器械在2.5%次氯酸钠冲洗下对患有坏死牙髓和根尖周炎的单根牙根管进行预备。从预备前后采集的样本中提取DNA，并使用定量实时聚合酶链反应对总细菌和链球菌进行定量分析。
所有初始样本细菌检测均为阳性，Reciproc组和BioRaCe组细菌细胞中位数分别为7.1×10⁵和1.31×10⁵。使用Reciproc和BioRaCe预备后，分别有16根（55%）和15根（50%）根管仍可检测到细菌，中位数计数分别为7.05×10²和6.03×10¹。两种系统在降低总细菌计数方面均非常有效（P <.001），且二者之间无显著差异（P >.05）。链球菌极为常见，两种系统均成功显著降低了其水平（P <.001）。
往复式单器械系统和旋转多器械系统在降低根尖周炎患牙根管内总细菌和链球菌计数方面均非常有效。无论使用哪种系统，约一半的牙齿仍可检测到细菌。
