Instituto de Química, Univ. Estadual Paulista-UNESP, Rua Prof. Francisco Degni, 55, Quitandinha, Araraquara, SP 14800-060, Brazil.
Faculdade de Ciências Farmacêuticas, Univ. Estadual Paulista-UNESP, Rod. Araraquara-Jaú km 1, Araraquara, SP 14801-903, Brazil.
Molecules. 2018 Mar 20;23(3):700. doi: 10.3390/molecules23030700.
Triterpenes are interesting compounds because they play an important role in cell homeostasis and a wide variety exhibiting defense functions is produced by plant secondary metabolism. Those same plant secondary metabolites also exhibit biological properties with promising therapeutic potential as anti-inflammatory and antitumor agents. Friedelin is a triterpene ketone with anti-inflammatory and gastroprotective activities and it is a precursor of relevant antitumor quinonemethides. Although many triterpene synthases have been described, only two friedelin synthases were characterized and there is no information about their genomic features and alleles. In the present work, we aimed to identify the gene and new isoforms of friedelin synthase in leaves to be functionally characterized in . The gene sequence analysis elucidated the exon/intron structure and confirmed the presence of single nucleotide polymorphisms with four non-synonymous mutations outside the active site of the enzyme. Therefore, two new isoforms were observed and the heterologous production of the enzymes in yeast showed similar production of friedelin. This first description of different alleles of the gene of friedelin synthase in can guide their validation as markers for friedelin-producer specimens.
三萜类化合物很有趣，因为它们在细胞内稳态中发挥着重要作用，而且植物次生代谢产物中也产生了多种多样具有防御功能的三萜类化合物。这些植物次生代谢产物还具有生物特性，具有作为抗炎和抗肿瘤剂的潜在治疗作用。羽扇醇是一种具有抗炎和胃保护活性的三萜酮，它是相关抗肿瘤醌甲烯醚的前体。尽管已经描述了许多三萜类化合物合成酶，但仅对两种羽扇醇合成酶进行了表征，并且关于它们的基因组特征和等位基因尚无信息。在本工作中，我们旨在鉴定叶片中羽扇醇合成酶的基因和新同工型，并在 中进行功能表征。基因序列分析阐明了外显子/内含子结构，并证实了酶活性位点外存在四个非同义突变的单核苷酸多态性。因此，观察到了两种新同工型，并且在酵母中异源生产的酶也产生了类似量的羽扇醇。这是首次在 中描述羽扇醇合成酶基因的不同等位基因，可以指导它们作为羽扇醇产生标本的标记进行验证。
