李痘病毒外壳蛋白区域在大肠杆菌中的表达。

Expression of the plum pox virus coat protein region in Escherichia coli.

作者信息

Mattanovich D, Himmler G, Laimer M, Maiss E, Regner F, Da Camara Machado A, Hanzer V, Casper R, Katinger H W

机构信息

Institute for Applied Microbiology, University of Agriculture, Vienna, Austria.

出版信息

Virus Genes. 1989 Mar;2(2):119-27. doi: 10.1007/BF00315256.

DOI:10.1007/BF00315256

PMID:2655276

Abstract

A cDNA complementary to the 3' end of plum pox virus (PPV) RNA was sequenced. The sequence was investigated for the presumable coat protein cistron by computer-aided translation. A fragment containing the stop codon of the polyprotein gene and a putative virus-specific protease cleavage site was subcloned into an E. coli expression vector. It is shown by immunological analysis that the coat protein cistron is located within the subcloned region.

摘要

对与李痘病毒（PPV）RNA 3' 末端互补的 cDNA 进行了测序。通过计算机辅助翻译对该序列进行了推测的衣壳蛋白顺反子研究。将包含多聚蛋白基因终止密码子和假定的病毒特异性蛋白酶切割位点的片段亚克隆到大肠杆菌表达载体中。免疫分析表明衣壳蛋白顺反子位于亚克隆区域内。