鸡胚脑以及小鼠神经母细胞瘤细胞N1E - 115和N - 18含有酸性脱氧核糖核酸酶的一种抑制剂。
Embryonic chicken brain and mouse neuroblastoma cells N1E-115 and N-18 contain an inhibitor of acid deoxyribonuclease.
作者信息
Bhattacharya P, Moskal J R, Basu S
出版信息
Proc Natl Acad Sci U S A. 1977 Mar;74(3):842-5. doi: 10.1073/pnas.74.3.842.
Abstract
Activity of a trypsin-sensitive acid deoxyribonuclease (DNase) inhibitor has been detected in 13- to 21-day-old embryonic chicken brains and in clonal lines of neuroblastoma cells (adrenergic, N1E-115, and neurotransmitter-inactive, N-18) grown for 48 hr after subculture. The activities of purified porcine and bovine spleen DNases were inhibited 60--75% in the presence of the inhibitor, whereas less than 10% inhibition was observed with purified pancreatic DNase. Activities of an acidic DNase and its inhibitor reached maxima in 21-day-old embryonic chicken brain. The proteins were separated by isoelectric focusing and affinity column chromatographic techniques. The pI values of the acid DNase and its inhibitor were 5.4 and 4.2, respectively.
摘要
在13至21日龄的鸡胚脑中以及在传代培养后生长48小时的神经母细胞瘤细胞克隆系（肾上腺素能的N1E-115和无神经递质活性的N-18）中检测到了一种对胰蛋白酶敏感的酸性脱氧核糖核酸酶（DNase）抑制剂的活性。在该抑制剂存在的情况下，纯化的猪和牛脾脏DNase的活性被抑制了60%至75%，而纯化的胰腺DNase的抑制率则低于10%。酸性DNase及其抑制剂的活性在21日龄的鸡胚脑中达到最大值。通过等电聚焦和亲和柱色谱技术对这些蛋白质进行了分离。酸性DNase及其抑制剂的pI值分别为5.4和4.2。
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