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新种,从北极高纬度冻原土壤中分离得到,以及对[相关物种1]和[相关物种2]的修订描述。

sp. nov., isolated from high-Arctic tundra soil, and emended descriptions of and .

作者信息

Kim TongRyul, Kim MyongChol, Kang OkChol, Jiang Fan, Chang Xulu, Liu Ping, Zhang Yumin, Da Xuyang, Zheng Congyi, Fang Chengxiang, Peng Fang

机构信息

China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.

College of Life Sciences, Kim Il Sung University, Pyongyang, DPR Korea.

出版信息

Int J Syst Evol Microbiol. 2016 Feb;66(2):609-615. doi: 10.1099/ijsem.0.000761. Epub 2015 Nov 11.

Abstract

A yellow, Gram-reaction-negative, non-motile, aerobic bacterium, designated D07, was isolated from a tundra soil near Ny-Ålesund, Svalbard archipelago, Norway (78° N). Growth occurred at 4-37 °C (optimum 28-30 °C) and at pH 6.0-9.0 (optimum pH 7.0-8.0). The strain produced flexirubin-type pigments. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D07 belonged to the genus in the family . The 16S rRNA gene sequence of this strain showed 93.83 and 93.31 % sequence similarity, respectively, to those of C26 and X-65. Strain D07 contained anteiso-C (25.91 %), iso-C (16.05 %), iso-C 3-OH (9.64 %), iso-C (9.42 %) and iso-C (7.36 %) as the predominant cellular fatty acids, MK-6 as the major respiratory quinone and phosphatidylethanolamine, five unknown aminolipids and three unknown lipids as the main polar lipids. The DNA G+C content was 49.3 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain D07 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D07 ( = CCTCC AB 2011160 = KCTC 42897). Emended descriptions of and are also provided.

摘要

从挪威斯瓦尔巴群岛新奥尔松(北纬78°)附近的苔原土壤中分离出一株黄色、革兰氏阴性、无运动性的需氧细菌,命名为D07。该菌在4 - 37°C(最适温度28 - 30°C)和pH 6.0 - 9.0(最适pH 7.0 - 8.0)条件下生长。该菌株产生柔韧红素类色素。基于16S rRNA基因序列的系统发育分析表明,菌株D07属于 科的 属。该菌株的16S rRNA基因序列与 C26和 X - 65的序列相似性分别为93.83%和93.31%。菌株D07的主要细胞脂肪酸包括anteiso - C(25.91%)、iso - C(16.05%)、iso - C 3 - OH(9.64%)、iso - C(9.42%)和iso - C(7.36%),主要呼吸醌为MK - 6,主要极性脂为磷脂酰乙醇胺、5种未知氨基脂和3种未知脂。DNA G + C含量为49.3 mol%。基于表型、化学分类和系统发育数据,菌株D07被认为代表 属的一个新物种,为此提出新物种名称 sp. nov.。模式菌株为D07(= CCTCC AB 2011160 = KCTC 42897)。同时还提供了 属和 属的修订描述。

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