Kim MyongChol, Pak SeHong, Rim SongGuk, Ren Lvzhi, Jiang Fan, Chang Xulu, Liu Ping, Zhang Yumin, Fang Chengxiang, Zheng Congyi, Peng Fang
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
College of Life Sciences, Kim Il Sung University, Pyongyang, DPR of Korea.
Int J Syst Evol Microbiol. 2015 Jun;65(Pt 6):1922-1928. doi: 10.1099/ijs.0.000202. Epub 2015 Mar 20.
A pale yellow, Gram-reaction-negative, non-motile, aerobic bacterium, designated MC 3726T, was isolated from a tundra soil near Ny-Ålesund, Svalbard Archipelago, Norway (78 °N). Growth occurred at 4-37 °C (optimum 25-30 °C) and at pH 5.0-9.0 (optimum pH 8.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MC 3726T belonged to the genus Luteolibacter in the family Verrucomicrobiaceae. The 16S rRNA gene sequence of this strain showed 93.18, 92.54 and 92.44 % similarity to those of Luteolibacter cuticulihirudinis E100T, Luteolibacter pohnpeiensis A4T-83T and Luteolibacter yonseiensis EBTL01T, respectively. The cell wall of strain MC 3726T contained meso-diaminopimelic acid as the diagnostic amino acid. Strain MC 3726T contained iso-C14:0 (38.28 %), C16:0 (15.89 %), C16:1ω9c (14.24 %), iso-C16:0 (10.42 %) and anteiso-C15:0 (5.75 %) as the predominant cellular fatty acids, MK-9 and MK-10 as the major respiratory quinones, and phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the main polar lipids. The DNA G+C content was 60.7 mol %. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain MC 3726T is considered to represent a novel species of the genus Luteolibacter, for which the name Luteolibacter arcticus sp. nov. is proposed. The type strain is MC 3726T ( = CCTCC AB 2014275T = LMG 28638T). An emended description of the genus Luteolibacter is also provided, along with emended descriptions of Luteolibacter cuticulihirudinis, Luteolibacter yonseiensis and Luteolibacter pohnpeiensis.
从挪威斯瓦尔巴群岛朗伊尔城（北纬78°）附近冻原土壤中分离出一株淡黄色、革兰氏阴性、无运动性、需氧的细菌，命名为MC 3726T。该菌在4-37 °C（最适温度25-30 °C）和pH 5.0-9.0（最适pH 8.0）条件下生长。基于16S rRNA基因序列的系统发育分析表明，菌株MC 3726T属于疣微菌科黄体杆菌属。该菌株的16S rRNA基因序列与表皮水蛭黄体杆菌E100T、波纳佩岛黄体杆菌A4T-83T和延世黄体杆菌EBTL01T的16S rRNA基因序列相似性分别为93.18%、92.54%和92.44%。菌株MC 3726T的细胞壁含有内消旋二氨基庚二酸作为诊断性氨基酸。菌株MC 3726T的主要细胞脂肪酸为异C14:0（38.28%）、C16:0（15.89%）、C16:1ω9c（14.24%）、异C16:0（10.42%）和anteiso-C15:0（5.75%），主要呼吸醌为MK-9和MK-10，主要极性脂为磷脂酰乙醇胺、磷脂酰甲基乙醇胺、磷脂酰甘油和二磷脂酰甘油。DNA G+C含量为60.7 mol%。基于表型、化学分类和系统发育数据，菌株MC 3726T被认为代表黄体杆菌属的一个新物种，为此提出了北极黄体杆菌新种（Luteolibacter arcticus sp. nov.）。模式菌株为MC 3726T（= CCTCC AB 2014275T = LMG 28638T）。同时还提供了黄体杆菌属的修订描述，以及表皮水蛭黄体杆菌、延世黄体杆菌和波纳佩岛黄体杆菌的修订描述。
