Peng Xiaoya, Zhang Yumin, Lu Yijing, Zhou Xueyin, Wei Zhourui, Sun Yulin, Kuang Chengjie, Lu Lu, Geng Yingchao, Qin Kun, Liu Jia, Peng Fang
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004740. Epub 2021 Mar 16.
A rod-shaped, yellow-pigmented, Gram-stain-negative, non-motile and aerobic bacterium, designated 7-3A, was isolated from soil from King George Island, maritime Antarctica, and subjected to a polyphasic taxonomic study. Growth occurred at 4-37 °C (optimum, 20°C) and at pH 5.0-9.0 (optimum, pH 7.0-8.0). Tolerance to NaCl was up to 4 % (w/v) with optimum growth in the absence of NaCl. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 7-3A represented a member of the family . Strain 7-3A showed the highest sequence similarities with HMD 1043 (96.65 %), NCTC 13525 (96.53 %), DSM 23145 (96.27 %), LMG 24720 (96.13 %) and DSM 17048 (96.06 %). A whole genome-level comparison of 7-3A with DSM 17048, LMG 24720, DSM 23145, and DSM 21579 revealed average nucleotide identity (ANI) values of 79.03, 82.25, 78.12, and 74.42 %, respectively. The major respiratory isoprenoid quinone was identified as MK-6 and a few ubiquinones Q-10 were identified. In addition, flexirubin-type pigments were absent. The polar lipid profile of 7-3A was found to contain one phosphatidylethanolamine, six unidentified aminolipids (AL) and two unidentified lipids (L). The G+C content of the genomic DNA was determined to be 34.54 mol%. The main fatty acids were iso-C, summed feature 9 (comprising iso-Cω9 and/or C 10-methyl), anteiso-C, iso-C and summed feature 3 (comprising Cω7 and/or Cω6). On the basis of the evidence presented in this study, a novel species of the genus , sp. nov., is proposed, with the type strain 7-3A (=CCTCC AB 2016141= KCTC 52492). Emended descriptions of , , and are also given.
从南极海洋乔治王岛的土壤中分离出一株杆状、产黄色色素、革兰氏染色阴性、无运动性的需氧细菌，命名为7-3A，并对其进行了多相分类研究。该菌在4-37°C（最适温度20°C）和pH 5.0-9.0（最适pH 7.0-8.0）条件下生长。对NaCl的耐受性高达4%（w/v），在无NaCl条件下生长最佳。基于16S rRNA基因序列的系统发育分析结果表明，菌株7-3A属于该科的一个成员。菌株7-3A与HMD 1043（96.65%）、NCTC 13525（96.53%）、DSM 23145（96.27%）、LMG 24720（96.13%）和DSM 17048（96.06%）的序列相似性最高。对7-3A与DSM 17048、LMG 24720、DSM 23145和DSM 21579进行全基因组水平比较，结果显示平均核苷酸同一性（ANI）值分别为79.03%、82.25%、78.12%和74.42%。主要呼吸类异戊二烯醌被鉴定为MK-6，还鉴定出少量泛醌Q-10。此外，未检测到柔韧红素类色素。发现7-3A的极性脂质谱包含一种磷脂酰乙醇胺、六种未鉴定的氨基脂质（AL）和两种未鉴定的脂质（L）。基因组DNA的G+C含量测定为34.54 mol%。主要脂肪酸为异-C、总和特征9（包括异-Cω9和/或C 10-甲基）、anteiso-C、异-C和总和特征3（包括Cω7和/或Cω6）。基于本研究提供的证据，提出了该属的一个新物种，新物种名为sp. nov.，模式菌株为7-3A（=CCTCC AB 2016141= KCTC 52492）。同时还给出了对该属、相关属等的修订描述。
