• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大肠杆菌dnaT基因功能是pBR322质粒复制所必需的，但不是R1质粒复制所必需的。

Escherichia coli dnaT gene function is required for pBR322 plasmid replication but not for R1 plasmid replication.

作者信息

Masai H, Arai K

机构信息

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304.

出版信息

J Bacteriol. 1989 Jun;171(6):2975-80. doi: 10.1128/jb.171.6.2975-2980.1989.

DOI:10.1128/jb.171.6.2975-2980.1989
PMID:2656633
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC210003/
Abstract

Plasmid pBR322 was unable to replicate in a temperature-sensitive dnaT1 strain at a nonpermissive temperature, whereas a pBR322-derived plasmid carrying the wild-type dnaT+ gene was able to replicate under the same conditions. In contrast to pBR322, plasmid R1 could replicate in the dnaT1 strain at a nonpermissive temperature. In keeping with this finding, in vitro replication of plasmid R1 did not require DnaT protein.

摘要

质粒pBR322在非允许温度下无法在温度敏感型dnaT1菌株中复制，而携带野生型dnaT+基因的pBR322衍生质粒在相同条件下能够复制。与pBR322不同，质粒R1在非允许温度下能够在dnaT1菌株中复制。与这一发现一致的是，质粒R1的体外复制不需要DnaT蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38ba/210003/e2ebdcbc505e/jbacter00172-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38ba/210003/9fcdc78dd921/jbacter00172-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38ba/210003/e2ebdcbc505e/jbacter00172-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38ba/210003/9fcdc78dd921/jbacter00172-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38ba/210003/e2ebdcbc505e/jbacter00172-0079-a.jpg

相似文献

1
Escherichia coli dnaT gene function is required for pBR322 plasmid replication but not for R1 plasmid replication.大肠杆菌dnaT基因功能是pBR322质粒复制所必需的，但不是R1质粒复制所必需的。
J Bacteriol. 1989 Jun;171(6):2975-80. doi: 10.1128/jb.171.6.2975-2980.1989.
2
Initiation of lagging-strand synthesis for pBR322 plasmid DNA replication in vitro is dependent on primosomal protein i encoded by dnaT.体外pBR322质粒DNA复制中后随链合成的起始依赖于由dnaT编码的引发体蛋白I。
J Biol Chem. 1988 Oct 15;263(29):15016-23.
3
Cloning of the Escherichia coli gene for primosomal protein i: the relationship to dnaT, essential for chromosomal DNA replication.大肠杆菌引发体蛋白i基因的克隆：与染色体DNA复制所必需的dnaT的关系。
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1256-60. doi: 10.1073/pnas.83.5.1256.
4
Inactivation of the replication-termination system affects the replication mode and causes unstable maintenance of plasmid R1.
Mol Microbiol. 1997 May;24(4):723-35. doi: 10.1046/j.1365-2958.1997.3791747.x.
5
A dnaT mutant with phenotypes similar to those of a priA2::kan mutant in Escherichia coli K-12.在大肠杆菌K-12中，一个具有与priA2::kan突变体相似表型的dnaT突变体。
Genetics. 2004 Jun;167(2):569-78. doi: 10.1534/genetics.103.025296.
6
Requirement of the Escherichia coli dnaA gene function for ori-2-dependent mini-F plasmid replication.大肠杆菌dnaA基因功能对ori-2依赖性微小F质粒复制的需求。
J Bacteriol. 1987 Apr;169(4):1724-30. doi: 10.1128/jb.169.4.1724-1730.1987.
7
Regulation of replication of plasmid pBR322 in amino acid-starved Escherichia coli strains.氨基酸饥饿型大肠杆菌菌株中质粒pBR322复制的调控
Mol Gen Genet. 1994 May 25;243(4):374-8. doi: 10.1007/BF00280467.
8
Rifampin-resistant replication of pBR322 derivatives in Escherichia coli cells induced for the SOS response.在诱导产生SOS反应的大肠杆菌细胞中，pBR322衍生物的耐利福平复制
J Bacteriol. 1991 Aug;173(15):4736-41. doi: 10.1128/jb.173.15.4736-4741.1991.
9
Rifampicin-induced replication of the plasmid pBR322 in Escherichia coli strains carrying dnaA mutations.利福平诱导携带dnaA突变的大肠杆菌菌株中质粒pBR322的复制。
Mol Gen Genet. 1983;190(2):326-30. doi: 10.1007/BF00330659.
10
Control of plasmid R1 replication: functions involved in replication, copy number control, incompatibility, and switch-off of replication.质粒R1复制的控制：涉及复制、拷贝数控制、不相容性及复制关闭的功能
J Bacteriol. 1980 Jan;141(1):111-20. doi: 10.1128/jb.141.1.111-120.1980.

引用本文的文献

1
Structural insight into the DNA-binding mode of the primosomal proteins PriA, PriB, and DnaT.引发体蛋白PriA、PriB和DnaT与DNA结合模式的结构解析
Biomed Res Int. 2014;2014:195162. doi: 10.1155/2014/195162. Epub 2014 Jul 21.
2
Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode.DnaT84 - 153 - dT10单链DNA复合物的晶体结构揭示了一种新型的单链DNA结合模式。
Nucleic Acids Res. 2014 Aug;42(14):9470-83. doi: 10.1093/nar/gku633. Epub 2014 Jul 22.
3
The rcbA gene product reduces spontaneous and induced chromosome breaks in Escherichia coli.

本文引用的文献

1
Expression of a DNA strand initiation sequence of ColE1 plasmid in a single-stranded DNA phage.ColE1质粒的DNA链起始序列在单链DNA噬菌体中的表达。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6566-70. doi: 10.1073/pnas.77.11.6566.
2
Purification and properties of Escherichia coli protein i, a prepriming protein in phi X174 DNA replication.大肠杆菌蛋白I的纯化及性质，X174噬菌体DNA复制中的一种引发前体蛋白
J Biol Chem. 1981 May 25;256(10):5281-6.
3
An amber replication mutant of F plasmid mapped in the minimal replication region.F质粒的一个琥珀复制突变体定位在最小复制区域。
rcbA 基因产物减少大肠杆菌中自发和诱导的染色体断裂。
J Bacteriol. 2012 May;194(9):2152-64. doi: 10.1128/JB.06390-11. Epub 2012 Feb 17.
4
Generic plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes.包含低代谢负担种子库和补料分批发酵工艺的通用质粒DNA生产平台。
Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43. doi: 10.1002/bit.22347.
5
A family of stability determinants in pathogenic bacteria.致病细菌中的一类稳定性决定因素。
J Bacteriol. 1998 Dec;180(23):6415-8. doi: 10.1128/JB.180.23.6415-6418.1998.
6
Primosome assembly site in Bacillus subtilis.枯草芽孢杆菌中的引发体装配位点。
EMBO J. 1995 Jun 1;14(11):2642-50. doi: 10.1002/j.1460-2075.1995.tb07262.x.
7
Escherichia coli PriA protein is essential for inducible and constitutive stable DNA replication.大肠杆菌PriA蛋白对于诱导型和组成型稳定DNA复制至关重要。
EMBO J. 1994 Nov 15;13(22):5338-45. doi: 10.1002/j.1460-2075.1994.tb06868.x.
8
Replication deficiencies in priA mutants of Escherichia coli lacking the primosomal replication n' protein.缺乏引发体复制n'蛋白的大肠杆菌priA突变体中的复制缺陷。
Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3029-32. doi: 10.1073/pnas.88.8.3029.
Mol Gen Genet. 1983;191(2):231-7. doi: 10.1007/BF00334819.
4
Definition of oriR, the minimum DNA segment essential for initiation of R1 plasmid replication in vitro.oriR的定义，即体外R1质粒复制起始所必需的最小DNA片段。
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6814-8. doi: 10.1073/pnas.80.22.6814.
5
n' Protein activator sites of plasmid pBR322 are not essential for its DNA replication.质粒pBR322的n'蛋白激活位点对其DNA复制并非必需。
J Mol Biol. 1983 Jul 5;167(3):751-6. doi: 10.1016/s0022-2836(83)80108-9.
6
Physical map of a 470 x 10(3) base-pair region flanking the terminus of DNA replication in the Escherichia coli K12 genome.大肠杆菌K12基因组中DNA复制终点侧翼一个470×10³碱基对区域的物理图谱。
J Mol Biol. 1982 Jan 5;154(1):1-20. doi: 10.1016/0022-2836(82)90413-2.
7
Control of replication of bacterial plasmids: genetics, molecular biology, and physiology of the plasmid R1 system.细菌质粒复制的控制：质粒R1系统的遗传学、分子生物学及生理学
Plasmid. 1984 Sep;12(2):71-90. doi: 10.1016/0147-619x(84)90054-4.
8
Regulation of plasmid replication.质粒复制的调控
Microbiol Rev. 1984 Mar;48(1):1-23. doi: 10.1128/mr.48.1.1-23.1984.
9
Identification of two Escherichia coli factor Y effector sites near the origins of replication of the plasmids (ColE1 and pBR322.在质粒（ColE1和pBR322）复制起点附近鉴定出两个大肠杆菌Y因子效应位点。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6521-5. doi: 10.1073/pnas.77.11.6521.
10
Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication.大肠杆菌素E1质粒DNA在细胞提取物中的复制。复制起点及方向
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260-4. doi: 10.1073/pnas.71.6.2260.