广义单颗粒冷冻电镜——历史视角
Generalized single-particle cryo-EM--a historical perspective.
作者信息
Frank Joachim
机构信息
HHMI, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA Department of Biological Sciences, Columbia University, New York, NY, USA
出版信息
Microscopy (Oxf). 2016 Feb;65(1):3-8. doi: 10.1093/jmicro/dfv358. Epub 2015 Nov 12.
Abstract
This is a brief account of the earlier history of single-particle cryo-EM of biological molecules lacking internal symmetry, which goes back to the mid-seventies. The emphasis of this review is on the mathematical concepts and computational approaches. It is written as the field experiences a turning point in the wake of the introduction of digital cameras capable of single electron counting, and near-atomic resolution can be reached even for smaller molecules.
摘要
这是一篇关于缺乏内部对称性的生物分子单颗粒冷冻电镜早期历史的简要叙述，这段历史可以追溯到20世纪70年代中期。本综述的重点是数学概念和计算方法。撰写此文时，该领域因能够进行单电子计数的数码相机的引入而经历了一个转折点，即使对于较小的分子也能达到近原子分辨率。
相似文献
1
Generalized single-particle cryo-EM--a historical perspective.广义单颗粒冷冻电镜——历史视角
Microscopy (Oxf). 2016 Feb;65(1):3-8. doi: 10.1093/jmicro/dfv358. Epub 2015 Nov 12.
2
Visualization of biological macromolecules at near-atomic resolution: cryo-electron microscopy comes of age.近原子分辨率下生物大分子的可视化：冷冻电子显微镜走向成熟。
Acta Crystallogr F Struct Biol Commun. 2019 Jan 1;75(Pt 1):3-11. doi: 10.1107/S2053230X18015133.
3
Cryo-EM Structure Determination Using Segmented Helical Image Reconstruction.使用分段螺旋图像重建进行冷冻电镜结构测定
Methods Enzymol. 2016;579:307-28. doi: 10.1016/bs.mie.2016.05.034. Epub 2016 Jun 28.
4
Single-particle cryo-electron microscopy of macromolecular complexes.大分子复合物的单颗粒冷冻电子显微镜技术
Microscopy (Oxf). 2016 Feb;65(1):9-22. doi: 10.1093/jmicro/dfv366. Epub 2015 Nov 25.
5
[A review of automatic particle recognition in Cryo-EM images].[冷冻电镜图像中自动粒子识别的综述]
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Oct;27(5):1178-82.
6
Challenges and opportunities in cryo-EM single-particle analysis.冷冻电镜单颗粒分析的挑战与机遇。
J Biol Chem. 2019 Mar 29;294(13):5181-5197. doi: 10.1074/jbc.REV118.005602. Epub 2019 Feb 25.
7
Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM.电子计数和束流诱导运动校正可实现近原子分辨率的单颗粒冷冻电镜。
Nat Methods. 2013 Jun;10(6):584-90. doi: 10.1038/nmeth.2472. Epub 2013 May 5.
8
Single-particle cryo-EM data acquisition by using direct electron detection camera.使用直接电子检测相机进行单颗粒冷冻电镜数据采集。
Microscopy (Oxf). 2016 Feb;65(1):35-41. doi: 10.1093/jmicro/dfv355. Epub 2015 Nov 6.
9
Refinement of Atomic Structures Against cryo-EM Maps.基于冷冻电镜图谱对原子结构的优化
Methods Enzymol. 2016;579:277-305. doi: 10.1016/bs.mie.2016.05.033. Epub 2016 Jun 24.
10
Cryo-electron microscopy and the amazing race to atomic resolution.冷冻电子显微镜与迈向原子分辨率的惊人竞赛。
Biochemistry. 2015 May 26;54(20):3133-41. doi: 10.1021/acs.biochem.5b00114. Epub 2015 May 14.
引用本文的文献
1
Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines.用于大分子机器的电子冷冻显微镜样品制备。
Adv Exp Med Biol. 2024;3234:173-190. doi: 10.1007/978-3-031-52193-5_12.
2
Recovery of Conformational Continuum From Single-Particle Cryo-EM Images: Optimization of ManifoldEM Informed by Ground Truth.从单颗粒冷冻电镜图像中恢复构象连续体：基于真实数据的ManifoldEM优化
IEEE Trans Comput Imaging. 2022;8:462-478. doi: 10.1109/tci.2022.3174801. Epub 2022 May 12.
3
A cryo-TSEM with temperature cycling capability allows deep sublimation of ice to uncover fine structures in thick cells.具有温度循环能力的 cryo-TSEM 允许冰的深度升华，以揭示厚细胞中的精细结构。
Sci Rep. 2021 Nov 1;11(1):21406. doi: 10.1038/s41598-021-00979-z.
4
Cryo-EM is a powerful tool, but helical applications can have pitfalls.冷冻电镜是一种强大的工具，但螺旋应用可能存在一些陷阱。
Soft Matter. 2021 Mar 28;17(12):3291-3293. doi: 10.1039/d1sm00282a. Epub 2021 Mar 17.
5
Advances in integrative structural biology: Towards understanding protein complexes in their cellular context.整合结构生物学进展：在细胞环境中理解蛋白质复合物
Comput Struct Biotechnol J. 2020 Dec 3;19:214-225. doi: 10.1016/j.csbj.2020.11.052. eCollection 2021.
6
POLARIS: Path of Least Action Analysis on Energy Landscapes.北极星：能量景观上的最小作用量路径分析
J Chem Inf Model. 2020 May 26;60(5):2581-2590. doi: 10.1021/acs.jcim.9b01108. Epub 2020 Feb 20.
7
The Evolution and the Advantages of MicroED.微晶电子衍射的发展与优势
Front Mol Biosci. 2018 Dec 12;5:114. doi: 10.3389/fmolb.2018.00114. eCollection 2018.
8
Ryanodine Receptor Structure and Function in Health and Disease.健康与疾病状态下的兰尼碱受体结构与功能
Subcell Biochem. 2018;87:329-352. doi: 10.1007/978-981-10-7757-9_11.
9
Dawning of a new era in TRP channel structural biology by cryo-electron microscopy.冷冻电镜技术开启 TRP 通道结构生物学新纪元。
Pflugers Arch. 2018 Feb;470(2):213-225. doi: 10.1007/s00424-018-2107-2. Epub 2018 Jan 17.
10
The translation elongation cycle-capturing multiple states by cryo-electron microscopy.通过冷冻电子显微镜捕获翻译延伸周期的多个状态。
Philos Trans R Soc Lond B Biol Sci. 2017 Mar 19;372(1716). doi: 10.1098/rstb.2016.0180.
本文引用的文献
1
Breaking the Crystal Ceiling.
ACS Cent Sci. 2015 Sep 23;1(6):283-5. doi: 10.1021/acscentsci.5b00295. Epub 2015 Sep 8.
2
Dynamical features of the Plasmodium falciparum ribosome during translation.恶性疟原虫核糖体在翻译过程中的动力学特征。
Nucleic Acids Res. 2015 Dec 2;43(21):10515-24. doi: 10.1093/nar/gkv991. Epub 2015 Oct 1.
3
Activation of GTP hydrolysis in mRNA-tRNA translocation by elongation factor G.延伸因子G在mRNA-tRNA易位过程中激活GTP水解。
Sci Adv. 2015 May 1;1(4). doi: 10.1126/sciadv.1500169.
4
Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity.冷冻电镜：一种用于可视化大分子复杂性的独特工具。
Mol Cell. 2015 May 21;58(4):677-89. doi: 10.1016/j.molcel.2015.02.019.
5
Cryo-electron microscopy and the amazing race to atomic resolution.冷冻电子显微镜与迈向原子分辨率的惊人竞赛。
Biochemistry. 2015 May 26;54(20):3133-41. doi: 10.1021/acs.biochem.5b00114. Epub 2015 May 14.
6
2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor.与一种细胞渗透性抑制剂结合的β-半乳糖苷酶的2.2埃分辨率冷冻电镜结构。
Science. 2015 Jun 5;348(6239):1147-51. doi: 10.1126/science.aab1576. Epub 2015 May 7.
7
A primer to single-particle cryo-electron microscopy.单颗粒冷冻电子显微镜入门
Cell. 2015 Apr 23;161(3):438-449. doi: 10.1016/j.cell.2015.03.050.
8
Structure of the E. coli ribosome-EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM.大肠杆菌核糖体-EF-Tu 复合物的结构通过 Cs 校正的 cryo-EM 解析至 <3Å 分辨率。
Nature. 2015 Apr 23;520(7548):567-70. doi: 10.1038/nature14275. Epub 2015 Feb 23.
9
Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine.与抗疟原虫药物吐根碱结合的恶性疟原虫80S核糖体的冷冻电镜结构。
Elife. 2014 Jun 9;3:e03080. doi: 10.7554/eLife.03080.
10
Structure of the yeast mitochondrial large ribosomal subunit.酵母线粒体大亚基的结构。
Science. 2014 Mar 28;343(6178):1485-1489. doi: 10.1126/science.1249410.
Zotero 插件