利用DNA适配体发现癌症生物标志物。
Cancer biomarker discovery using DNA aptamers.
作者信息
Jin Cheng, Qiu Liping, Li Jin, Fu Ting, Zhang Xiaobing, Tan Weihong
机构信息
Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Molecular Engineering for Theranostics, Hunan University, Changsha, 410082, China.
Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Molecular Engineering for Theranostics, Hunan University, Changsha, 410082, China and Center for Research at Bio/nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Health Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, USA.
出版信息
Analyst. 2016 Jan 21;141(2):461-6. doi: 10.1039/c5an01918d.
Biomarkers are signature molecules able to indicate specific physiological states of cells. Identification of reliable biomarkers is essential for early diagnosis and adaptive treatment of diseases, especially cancer. Aptamers are single-stranded oligonucleotides generated by an in vitro screening method called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). They can recognize their cognate targets with selectivity and affinity comparable to protein antibodies. In addition, aptamers have superiorities including easy synthesis, high chemical stability, convenient modification and flexible design. As such, these DNA molecules show great promise as powerful molecular probes for biomarker discovery and biomarker-based clinical applications. Using complex samples as targets, a panel of aptamers can be systematically generated for comprehensive recognition of disease-specific proteins, which can potentially serve as biomarkers. This review describes the current methods for biomarker discovery using aptamers.
生物标志物是能够指示细胞特定生理状态的标志性分子。鉴定可靠的生物标志物对于疾病尤其是癌症的早期诊断和适应性治疗至关重要。适体是通过一种称为指数富集配体系统进化（SELEX）的体外筛选方法产生的单链寡核苷酸。它们能够以与蛋白质抗体相当的选择性和亲和力识别其同源靶标。此外，适体具有易于合成、化学稳定性高、修饰方便和设计灵活等优势。因此，这些DNA分子作为用于生物标志物发现和基于生物标志物的临床应用的强大分子探针具有巨大潜力。以复杂样品为靶标，可以系统地生成一组适体，用于全面识别疾病特异性蛋白质，这些蛋白质有可能作为生物标志物。本文综述了目前使用适体发现生物标志物的方法。
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