Wang Jian, Chang Yong, Zhang Pu, Lie Shao Qing, Gao Peng Fei, Huang Cheng Zhi
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University) Ministry of Education, College of Pharmaceutical Science, Southwest University, Chongqing 400715, China.
Chongqing Key Laboratory of Biomedical Analysis (Southwest University), Chongqing Science & Technology Commission, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
Analyst. 2015 Dec 21;140(24):8194-200. doi: 10.1039/c5an01975c.
It is of great significance to sense clioquinol (CQ) in a simple and fast way because of its potential application in the treatment of neurodegenerative diseases. In this contribution, we proposed a Cu(2+)-mediated fluorescence switchable strategy to detect CQ by taking bovine serum albumin (BSA) protected gold nanoclusters (AuNCs) as probes. It was found that the strong red fluorescence of BSA-protected AuNCs at 610 nm could be effectively quenched by Cu(2+) (off state) and reversibly recovered by CQ (on state) owing to the specific coordination of CQ and Cu(2+). Under the optimal conditions, there was a good linear relationship between the off-on efficiency (Eoff-on) and the amount of CQ in the range of 1-12 μM (R(2) = 0.9902), with a detection limit of 0.63 μM (3σ). The "turn off-on" mode and the fast and unique complexation of CQ and Cu(2+) endow AuNCs with high specificity for CQ sensing. The proposed strategy is label-free, fast and selective, which is applicable to the analysis of CQ in cream with satisfactory results.
由于氯碘羟喹（CQ）在神经退行性疾病治疗中的潜在应用，以简单快速的方式检测它具有重要意义。在本研究中，我们提出了一种铜离子（Cu(2+)）介导的荧光开关策略，以牛血清白蛋白（BSA）保护的金纳米簇（AuNCs）为探针来检测CQ。研究发现，由于CQ与Cu(2+)的特异性配位作用，BSA保护的AuNCs在610 nm处的强红色荧光可被Cu(2+)有效猝灭（关闭状态），并被CQ可逆恢复（开启状态）。在最佳条件下，关闭-开启效率（Eoff-on）与CQ含量在1-12 μM范围内呈良好的线性关系（R(2)=0.9902），检测限为0.63 μM（3σ）。“关闭-开启”模式以及CQ与Cu(2+)快速且独特的络合作用赋予了AuNCs对CQ传感的高特异性。所提出的策略无需标记、快速且具有选择性，适用于乳膏中CQ的分析，结果令人满意。
