Ghasemzadeh Jalal, Talebi Ali Reza, Khalili Mohammad Ali, Fesahat Farzaneh, Halvaei Iman, Nabi Ali, Ashourzadeh Sareh
International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Iran J Reprod Med. 2015 Aug;13(8):495-502.
Globozoospermia is a severe form of teratozoospermia (incidence < 0.1%) in infertile men that is characterized by round headed sperm and acrosomeless in semen.
To compare the semen parameters, protamine deficiency, and apoptosis in ejaculated spermatozoa between globozoospermic and normozoospermic men.
Thirty six semen samples were divided into two groups including 15 infertile men with total globozoospermic (> 90% round-headed sperm) and 21 healthy donors with normal spermograms as controls. Semen analysis was performed according to World Health Organization criteria (2010). Sperm protamine deficiency was assessed using Chromomycin A3 (CMA3) staining and the rate of apoptotic spermatozoa was evaluated with TUNEL assay.
Sperm concentration, motility, and normal morphology in globozoospermic men were significantly decreased compared with controls (p<0.05). The rate of CMA3-reacted spermatozoa (CMA3+) in globozoospermic men was higher than controls (65.93 ± 11.77 vs. 21.24 ± 7.37, respectively, p<0.0001). The rate of apoptotic spermatozoa (TUNEL positive) were significantly increased in globozoospermic cases with respect to the controls (17.60 ± 10.72 and 5.95 ± 3.02, respectively, p<0.0001). There was no significant correlation between sperm protamine deficiency and apoptosis in globozoospermic men.
Globozoospermic samples contain a higher proportion of spermatozoa with abnormal chromatin packaging and DNA fragmentation than normozoospermic samples. Therefore, in addition to absence of acrosome in the spermatozoa of globozoospermic patients, the high percentage of spermatozoa with immature chromatin and apoptotic marker may be considered as the other etiologies of infertility in these patients.
圆头精子症是不育男性中严重的畸形精子症形式（发病率<0.1%），其特征是精液中出现圆头精子且无顶体。
比较圆头精子症男性与正常精子症男性射出精子的精液参数、鱼精蛋白缺乏情况及细胞凋亡情况。
36份精液样本分为两组，包括15名患有完全圆头精子症（>90%圆头精子）的不育男性和21名精子图谱正常的健康供者作为对照。根据世界卫生组织标准（2010年）进行精液分析。使用嗜铬霉素A3（CMA3）染色评估精子鱼精蛋白缺乏情况，并用TUNEL法评估凋亡精子的比例。
与对照组相比，圆头精子症男性的精子浓度、活力和正常形态显著降低（p<0.05）。圆头精子症男性中CMA3反应精子（CMA3+）的比例高于对照组（分别为65.93±11.77和21.24±7.37，p<0.0001）。圆头精子症患者中凋亡精子（TUNEL阳性）的比例相对于对照组显著增加（分别为17.60±10.72和5.95±3.02，p<0.0001）。圆头精子症男性中精子鱼精蛋白缺乏与细胞凋亡之间无显著相关性。
与正常精子症样本相比，圆头精子症样本中染色质包装异常和DNA片段化的精子比例更高。因此，除了圆头精子症患者精子中无顶体外，染色质未成熟和凋亡标记的精子比例高也可能被视为这些患者不育的其他病因。
