Hosseinifar H, Yazdanikhah S, Modarresi T, Totonchi M, Sadighi Gilani M A, Sabbaghian M
Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Andrology. 2015 May;3(3):526-31. doi: 10.1111/andr.12030. Epub 2015 Apr 10.
The absence of the acrosome causes the situation which is called globozoospermia. There are a few studies, mostly as case reports, about correlation between levels of sperm DNA damage in patients with total round-headed spermatozoa. We investigated this correlation as well as CMA3 positive spermatozoa in 20 globozoospermic men (with more than 90% round-headed spermatozoa) attending to Royan Institute. Semen samples divided into three parts to semen analysis, to measure DNA fragmentation index (DFI) using sperm chromatin structure assay (SCSA) and to detect CMA3(+) sperm cells by chromomycin A3 staining and fluorescent microscopy. Our results showed that there were significant differences in sperm concentration, total sperm motility, and normal morphology between patients and controls group (p < 0.001). Moreover, the average of DFI and CMA3 positive spermatozoa in patients group significantly increases compared with control group (p < 0.001). A significant correlation between DFI and CMA3(+) in total population was also detected in patients group (r = 0.45, p = 0.046). To our knowledge, this is the largest study about correlation between DNA damage levels and CMA3 positive spermatozoa with round head sperm cells in total globozoospermic men. It seems that the increase in DNA damage may be because of defective sperm DNA compaction, as we detected CMA3 positive sperm cells in these patients.
顶体缺失会导致一种名为圆头精子症的情况。关于完全圆头精子症患者精子DNA损伤水平之间的相关性，有一些研究，大多是病例报告。我们对20名前往罗扬研究所就诊的圆头精子症男性（圆头精子比例超过90%）的这种相关性以及CMA3阳性精子进行了研究。精液样本分为三部分，分别用于精液分析、使用精子染色质结构分析（SCSA）测量DNA碎片化指数（DFI）以及通过放线菌素A3染色和荧光显微镜检测CMA3（+）精子细胞。我们的结果显示，患者组与对照组在精子浓度、总精子活力和正常形态方面存在显著差异（p < 0.001）。此外，患者组的DFI平均值和CMA3阳性精子与对照组相比显著增加（p < 0.001）。在患者组中还检测到总体人群中DFI与CMA3（+）之间存在显著相关性（r = 0.45，p = 0.046）。据我们所知，这是关于完全圆头精子症男性中DNA损伤水平与CMA3阳性圆头精子细胞之间相关性的最大规模研究。由于我们在这些患者中检测到了CMA3阳性精子细胞，DNA损伤的增加似乎可能是由于精子DNA压缩缺陷所致。
