Mou Yan, Xu Dan, Mao Ziling, Dong Xuejiao, Lin Fengke, Wang Ali, Lai Daowan, Zhou Ligang, Xie Bingyan
Department of Plant Pathology, College of Agronomy and Biotechnology, China Agronomy and Biotechnology, Beijing 100193, China.
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Molecules. 2015 Nov 12;20(11):20320-33. doi: 10.3390/molecules201119700.
The endophytic fungus Berkleasmium sp. Dzf12, isolated from Dioscorea zingiberensis, was found to produce palmarumycins C12 and C13 which possess a great variety of biological activities. Seven biocompatible water-immiscible organic solvents including n-dodecane, n-hexadecane, 1-hexadecene, liquid paraffin, dibutyl phthalate, butyl oleate and oleic acid were evaluated to improve palmarumycins C12 and C13 production in suspension culture of Berkleasmium sp. Dzf12. Among the chosen solvents both butyl oleate and liquid paraffin were the most effective to improve palmarumycins C12 and C13 production. The addition of dibutyl phthalate, butyl oleate and oleic acid to the cultures of Berkleasmium sp. Dzf12 significantly enhanced palmarumycin C12 production by adsorbing palmarumycin C12 into the organic phase. When butyl oleate was fed at 5% (v/v) in medium at the beginning of fermentation (day 0), the highest palmarumycin C12 yield (191.6 mg/L) was achieved, about a 34.87-fold increase in comparison with the control (5.3 mg/L). n-Dodecane, 1-hexadecene and liquid paraffin had a great influence on the production of palmarumycin C13. When liquid paraffin was added at 10% (v/v) in medium on day 3 of fermentation, the palmarumycin C13 yield reached a maximum value (134.1 mg/L), which was 4.35-fold that of the control (30.8 mg/L). Application of the aqueous-organic solvent system should be a simple and efficient process strategy for enhancing palmarumycin C12 and C13 production in liquid cultures of the endophytic fungus Berkleasmium sp. Dzf12.
从盾叶薯蓣中分离得到的内生真菌伯克氏菌属菌株Dzf12，被发现能产生具有多种生物活性的棕榈霉素C12和C13。评估了七种生物相容性的与水不混溶的有机溶剂，包括正十二烷、正十六烷、1-十六烯、液体石蜡、邻苯二甲酸二丁酯、油酸丁酯和油酸，以提高伯克氏菌属菌株Dzf12悬浮培养中棕榈霉素C12和C13的产量。在所选择的溶剂中，油酸丁酯和液体石蜡对提高棕榈霉素C12和C13的产量最有效。向伯克氏菌属菌株Dzf12的培养物中添加邻苯二甲酸二丁酯、油酸丁酯和油酸，通过将棕榈霉素C12吸附到有机相中，显著提高了棕榈霉素C12的产量。当在发酵开始时（第0天）在培养基中以5%（v/v）的比例加入油酸丁酯时，获得了最高的棕榈霉素C12产量（191.6 mg/L），与对照（5.3 mg/L）相比增加了约34.87倍。正十二烷、1-十六烯和液体石蜡对棕榈霉素C13的产量有很大影响。当在发酵第3天在培养基中以10%（v/v）的比例加入液体石蜡时，棕榈霉素C13产量达到最大值（134.1 mg/L），是对照（30.8 mg/L）的4.35倍。应用水-有机溶剂体系应该是一种简单有效的工艺策略，用于提高内生真菌伯克氏菌属菌株Dzf12液体培养中棕榈霉素C12和C13的产量。
