Luo Haiyu, Liu Hongwei, Cao Yuheng, Xu Dan, Mao Ziling, Mou Yan, Meng Jiajia, Lai Daowan, Liu Yang, Zhou Ligang
MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Molecules. 2014 Sep 10;19(9):14221-34. doi: 10.3390/molecules190914221.
Hyalodendriella sp. Ponipodef12, an endophytic fungus from the hybrid "Neva" of Populus deltoides × P. nigra, is a high producer of the bioactive dibenzo-α-pyrones botrallin and TMC-264. However, both the botrallin and TMC-264 produced by Hyalodendriella sp. Ponipodef12 were retained as both intracellular and extracellular products. The aim of this study was to evaluate an in situ macroporous resin adsorption for enhancement of botrallin and TMC-264 production in mycelial liquid culture of Hyalodendriella sp. Ponipodef12. Production of botrallin and TMC-264 was most effectively enhanced by macroporous resin DM-301 among the thirteen nonionic macroporous resins tested. The highest botrallin yield (51.47 mg/L, which was 2.29-fold higher than the control at 22.49 mg/L) was obtained by adding resin DM-301 at 4.38% (g/mL) to the culture broth on day 24 and allowing a period of 4 days for adsorption. The highest TMC-264 yield reached 47.74 mg/L, which was 11.76-fold higher than that of the control (4.06 mg/L), and was achieved by adding DM-301 resin at 4.38% (w/v) in the culture broth on day 24 and allowing a period of 6 days for adsorption. The results show that in situ resin adsorption is an effective strategy for enhancing production of botrallin and TMC-264, and also for facilitating their recovery from mycelial liquid culture of Hyalodendriella sp. Ponipodef12.
波氏透明枝孢菌Ponipodef12是从三角叶杨×黑杨的杂交种“涅瓦”中分离得到的一种内生真菌，是生物活性二苯并-α-吡喃酮博特拉林和TMC-264的高产菌株。然而，波氏透明枝孢菌Ponipodef12产生的博特拉林和TMC-264既存在于细胞内产物中，也存在于细胞外产物中。本研究的目的是评估原位大孔树脂吸附法，以提高波氏透明枝孢菌Ponipodef12菌丝体液体培养中博特拉林和TMC-264的产量。在所测试的13种非离子大孔树脂中，大孔树脂DM-301对博特拉林和TMC-264产量的提高效果最为显著。在第24天向培养液中添加4.38%（g/mL）的树脂DM-301并吸附4天，可获得最高的博特拉林产量（51.47 mg/L，比对照的22.49 mg/L高2.29倍）。最高的TMC-264产量达到47.74 mg/L，比对照（4.06 mg/L）高11.76倍，这是通过在第24天向培养液中添加4.38%（w/v）的DM-301树脂并吸附6天实现的。结果表明，原位树脂吸附是提高博特拉林和TMC-264产量以及促进从波氏透明枝孢菌Ponipodef12菌丝体液体培养物中回收它们的有效策略。
