Mou Yan, Luo Haiyu, Mao Ziling, Shan Tijiang, Sun Weibo, Zhou Kaiyi, Zhou Ligang
Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Int J Mol Sci. 2013 Jan 7;14(1):979-98. doi: 10.3390/ijms14010979.
The influences of eight metal ions (i.e., Na+, Ca2+, Ag+, Co2+, Cu2+, Al3+, Zn2+, and Mn4+) on mycelia growth and palmarumycins C(12) and C(13) production in liquid culture of the endophytic fungus Berkleasmium sp. Dzf12 were investigated. Three metal ions, Ca2+, Cu2+ and Al3+ were exhibited as the most effective to enhance mycelia growth and palmarumycin production. When calcium ion (Ca2+) was applied to the medium at 10.0 mmol/L on day 3, copper ion (Cu2+) to the medium at 1.0 mmol/L on day 3, aluminum ion (Al3+) to the medium at 2.0 mmol/L on day 6, the maximal yields of palmarumycins C(12) plus C(13) were obtained as 137.57 mg/L, 146.28 mg/L and 156.77 mg/L, which were 3.94-fold, 4.19-fold and 4.49-fold in comparison with that (34.91 mg/L) of the control, respectively. Al3+ favored palmarumycin C(12) production when its concentration was higher than 4 mmol/L. Ca2+ had an improving effect on mycelia growth of Berkleasmium sp. Dzf12. The combination effects of Ca2+, Cu2+ and Al3+ on palmarumycin C(13) production were further studied by employing a statistical method based on the central composite design (CCD) and response surface methodology (RSM). By solving the quadratic regression equation between palmarumycin C(13) and three metal ions, the optimal concentrations of Ca2+, Cu2+ and Al3+ in medium for palmarumycin C(13) production were determined as 7.58, 1.36 and 2.05 mmol/L, respectively. Under the optimum conditions, the predicted maximum palmarumycin C(13) yield reached 208.49 mg/L. By optimizing the combination of Ca2+, Cu2+ and Al3+ in medium, palmarumycin C(13) yield was increased to 203.85 mg/L, which was 6.00-fold in comparison with that (33.98 mg/L) in the original basal medium. The results indicate that appropriate metal ions (i.e., Ca2+, Cu2+ and Al3+) could enhance palmarumycin production. Application of the metal ions should be an effective strategy for palmarumycin production in liquid culture of the endophytic fungus Berkleasmium sp. Dzf12.
研究了8种金属离子（即Na⁺、Ca²⁺、Ag⁺、Co²⁺、Cu²⁺、Al³⁺、Zn²⁺和Mn⁴⁺）对内生真菌伯克利氏菌属Dzf12液体培养中菌丝体生长以及棕榈霉素C(12)和C(13)产量的影响。结果表明，Ca²⁺、Cu²⁺和Al³⁺这三种金属离子对促进菌丝体生长和棕榈霉素产量最为有效。当在第3天将钙离子（Ca²⁺）以10.0 mmol/L添加到培养基中、第3天将铜离子（Cu²⁺）以1.0 mmol/L添加到培养基中、第6天将铝离子（Al³⁺）以2.0 mmol/L添加到培养基中时，棕榈霉素C(12)和C(13)的最大产量分别达到137.57 mg/L、146.28 mg/L和156.77 mg/L，分别是对照（34.91 mg/L）的3.94倍、4.19倍和4.49倍。当Al³⁺浓度高于4 mmol/L时，有利于棕榈霉素C(12)的产生。Ca²⁺对伯克利氏菌属Dzf12的菌丝体生长有促进作用。采用基于中心复合设计（CCD）和响应面方法（RSM）的统计方法，进一步研究了Ca²⁺、Cu²⁺和Al³⁺对棕榈霉素C(13)产量的联合效应。通过求解棕榈霉素C(13)与三种金属离子之间的二次回归方程，确定培养基中用于生产棕榈霉素C(13)的Ca²⁺、Cu²⁺和Al³⁺的最佳浓度分别为7.58、1.36和2.05 mmol/L。在最佳条件下，预测的棕榈霉素C(13)最大产量达到208.49 mg/L。通过优化培养基中Ca²⁺、Cu²⁺和Al³⁺的组合，棕榈霉素C(13)产量提高到203.85 mg/L，是原始基础培养基中产量（33.98 mg/L）的6.00倍。结果表明，适当的金属离子（即Ca²⁺、Cu²⁺和Al³⁺）可提高棕榈霉素的产量。在内生真菌伯克利氏菌属Dzf12的液体培养中应用金属离子应该是提高棕榈霉素产量的有效策略。
