Yang Hui, Li Lie-Jun, Xie Long-Xu, Luo Zhao-Yun, Lu Min, Lin Min, Zheng Xiang-Bin, Huang Yue, Yang Li-Ye
Central Laboratory, Chaozhou Central Hospital Affiliated to Southern Medical University, No. 84 Huan-Cheng-Xi Road, Chaozhou, 521021, Guangdong, People's Republic of China.
Chaozhou Hybribio Limited Corporation, Chaozhou, Guangdong, People's Republic of China.
Arch Virol. 2016 Feb;161(2):449-54. doi: 10.1007/s00705-015-2673-y. Epub 2015 Nov 17.
In the present study, we describe the laboratory workflow and the clinical validation of a novel multiplex real-time PCR-based HPV assay in China. The cross-sectional validation analysis showed that this assay worked well for detection of 14 HR-HPV types and identification of HPV 16 and 18 in a single sensitive assay that is suitable for both clinical usage and high-throughput cervical screening purposes. We predict that this accurate, high-throughput and low-cost HPV assay can greatly reduce the heavy economic burden of HPV detection in China.
在本研究中，我们描述了中国一种基于多重实时PCR的新型HPV检测方法的实验室工作流程和临床验证。横断面验证分析表明，该检测方法在单次灵敏检测中能很好地检测14种高危型人乳头瘤病毒（HR-HPV）类型并鉴定HPV 16和18型，适用于临床应用和高通量宫颈癌筛查。我们预计，这种准确、高通量且低成本的HPV检测方法可大大减轻中国HPV检测的沉重经济负担。
