Carson Spencer, Wick Scott T, Carr Peter A, Wanunu Meni, Aguilar Carlos A
Department of Physics, Northeastern University , Boston, Massachusetts 02115, United States.
Massachusetts Institute of Technology Lincoln Laboratory, Massachusetts Institute of Technology , 244 Wood Street, Lexington, Massachusetts 02420, United States.
ACS Nano. 2015 Dec 22;9(12):12417-24. doi: 10.1021/acsnano.5b05782. Epub 2015 Nov 20.
Synthetic nucleic acids offer rich potential to understand and engineer new cellular functions, yet an unresolved limitation in their production and usage is deleterious products, which restrict design complexity and add cost. Herein, we employ a solid-state nanopore to differentiate molecules of a gene synthesis reaction into categories of correct and incorrect assemblies. This new method offers a solution that provides information on gene synthesis reactions in near-real time with higher complexity and lower costs. This advance can permit insights into gene synthesis reactions such as kinetics monitoring, real-time tuning, and optimization of factors that drive reaction-to-reaction variations as well as open venues between nanopore-sensing, synthetic biology, and DNA nanotechnology.
合成核酸为理解和设计新的细胞功能提供了巨大潜力，但在其生产和使用中一个尚未解决的限制是有害产物，这些产物限制了设计的复杂性并增加了成本。在此，我们利用固态纳米孔将基因合成反应的分子区分为正确和错误组装类别。这种新方法提供了一种解决方案，能够以更高的复杂性和更低的成本近乎实时地提供有关基因合成反应的信息。这一进展可以让人深入了解基因合成反应，如动力学监测、实时调整以及驱动反应间变化的因素的优化，还能在纳米孔传感、合成生物学和DNA纳米技术之间开辟新途径。
