Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.
1] Wyss Institute for Biologically Inspired Engineering, Boston, Massachusetts, USA. [2] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.
Nat Methods. 2014 May;11(5):499-507. doi: 10.1038/nmeth.2918.
For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the de novo synthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addition, we discuss emerging applications enabled by large-scale de novo DNA constructs, as well as the challenges and opportunities that lie ahead.
60 多年来，新 DNA 序列的合成生产帮助研究人员理解和设计生物学。在这里，我们总结了从头合成 DNA 的方法和注意事项，特别强调了最近允许大规模和低成本生产的技术。此外，我们还讨论了大规模从头 DNA 构建体带来的新应用，以及未来的挑战和机遇。
Zotero 插件
