低强度激光疗法对骨髓干细胞培养增殖作用的评估。
Evaluation of the Proliferative Effects Induced by Low-Level Laser Therapy in Bone Marrow Stem Cell Culture.
作者信息
Cavalcanti Marcos Fernando Xisto Braga, Maria Durvanei A, de Isla Natalia, Leal-Junior Ernesto Cesar Pinto, Joensen Jon, Bjordal Jan Magnus, Lopes-Martins Rodrigo A M B, Diomede Francesca, Trubiani Oriana, Frigo Lucio
机构信息
1 Postgraduate Department, Cruzeiro do Sul University São Paulo , SP, Brasil .
2 UMR-7365, Faculté de Médecine, CNRS-Université de Lorraine , Vandoeuvre-lés-Nancy, France .
出版信息
Photomed Laser Surg. 2015 Dec;33(12):610-6. doi: 10.1089/pho.2014.3864. Epub 2015 Nov 18.
OBJECTIVE
The objective of this study was to evaluate the effect of laser irradiation on dog bone marrow stem cells.
BACKGROUND DATA
Low doses of low-level red laser positively affect the viability of mesenchymal stem cells, and also increase proliferation.
METHODS
Low-level laser (wavelength, 660 nm; power output, 50 mW), was applied to dog bone marrow stem cell cultures (DBMSC). The energy densities delivered varied from 1 to 12J/cm(2). The effect of the laser irradiation was evaluated on cell proliferation measured with the MTT colorimetric test, cell cycle phase, and on lipidic peroxidation (free radical production).
RESULTS
The results indicate that laser irradiation to DBMSC did not change the morphology of the cells, but significantly increased their viability and the number of cells at the G2/M phase with 6, 10, and 12 J/cm(2). On the other hand, malonaldehyde production was significantly enhanced with 8 J/cm(2).
CONCLUSIONS
The parameters used to irradiate DBMSC increased significantly proliferation without producing high levels of reactive oxygen species (ROS).
目的
本研究的目的是评估激光照射对犬骨髓干细胞的影响。
背景数据
低剂量的低强度红色激光对间充质干细胞的活力有积极影响，并且还能促进其增殖。
方法
将低强度激光（波长660nm；输出功率50mW）应用于犬骨髓干细胞培养物（DBMSC）。所传递的能量密度在1至12J/cm²之间变化。通过MTT比色法测量细胞增殖、细胞周期阶段以及脂质过氧化（自由基产生）来评估激光照射的效果。
结果
结果表明，对DBMSC进行激光照射并未改变细胞形态，但在能量密度为6、10和12J/cm²时，显著提高了细胞活力以及处于G2/M期的细胞数量。另一方面，在能量密度为8J/cm²时，丙二醛产量显著增加。
结论
用于照射DBMSC的参数显著提高了细胞增殖，且未产生高水平的活性氧（ROS）。
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