基于纸的分析装置，用于无仪器检测唾液中的硫氰酸盐作为吸烟暴露的生物标志物。

Paper-based analytical device for instrumental-free detection of thiocyanate in saliva as a biomarker of tobacco smoke exposure.

机构信息

Analytical and Food Chemistry Department, Faculty of Chemistry, University of Vigo, Campus As Lagoas-Marcosende s/n, 36310 Vigo, Spain.

出版信息

Talanta. 2016 Jan 15;147:390-6. doi: 10.1016/j.talanta.2015.10.013. Epub 2015 Oct 20.

DOI:10.1016/j.talanta.2015.10.013

PMID:26592623

Abstract

This work describes a fast and simple assay for in situ detection of thiocyanate, i.e., a biomarker of tobacco smoke exposure, in human saliva. The assay is based on the formation of an iron(III)-thiocyanate colored complex in a paper-based sensing platform and subsequent image analysis using a scanner as detection device. Experimental parameters influencing the color intensity of the complex were fully evaluated, including the selection of detection conditions, type of paper substrate, test zone dimensions and composition as well as the stability of the paper-based device. Under optimal conditions, the detection limit was 0.06mM of thiocyanate, and the repeatability, expressed as relative standard deviation, was 3%. The proposed method, characterized by its simplicity, portability and low sample consumption, was applied to the detection of thiocyanate in a series of human saliva samples. Average thiocyanate levels in the ranges 0.28-0.87mM and 0.78-4.28mM were found for non-smokers and smokers, respectively. Recovery studies were carried out at two concentration levels, showing recovery values in the range of 96.1-103.6%.

摘要

这项工作描述了一种快速而简单的方法，用于在人唾液中现场检测硫氰酸盐，即烟草烟雾暴露的生物标志物。该方法基于在基于纸张的传感平台中形成铁（III）-硫氰酸盐有色络合物，然后使用扫描仪作为检测设备进行图像分析。实验参数全面评估了络合物颜色强度的影响，包括检测条件的选择、纸张基底的类型、测试区尺寸和组成以及基于纸张的设备的稳定性。在最佳条件下，检测限为 0.06mM 的硫氰酸盐，重复性（表示为相对标准偏差）为 3%。所提出的方法具有简单、便携和低样品消耗的特点，已应用于一系列人唾液样本中硫氰酸盐的检测。分别在非吸烟者和吸烟者中发现硫氰酸盐水平在 0.28-0.87mM 和 0.78-4.28mM 范围内。在两个浓度水平进行了回收率研究，回收率值在 96.1-103.6%范围内。