Khabour Omar F, Enaya Fatima M, Alzoubi Karem, Al-Azzam Sayer I
a Department of Medical Laboratory Sciences , Jordan University of Science and Technology , Irbid , Jordan .
b Biology Department , Faculty of Science, Taibah University , Almedina , Saudi Arabia , and.
Drug Chem Toxicol. 2016;39(3):303-6. doi: 10.3109/01480545.2015.1113988. Epub 2015 Nov 24.
Norcantharidin (NCTD) is currently used in the treatment of several cancers such as leukemia, melanoma and hepatoma. The mechanism of action of NCTD is suggested to involve induction of apoptosis of cancer cells via production of reactive oxygen species. In this study, the genotoxic effect of different concentrations of NCTD (1, 10 and 20 μm) in human lymphocytes was investigated using sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs) assays. The results revealed that NCTD significantly increased the rate of SCEs (p < 0.05) in a dose-dependent manner. In addition, NCTD significantly increased the number of high-frequency cells (SCEs ≥ 8, p < 0.05). However, NCTD did not have any significant effect on the rate of CAs (p > 0.05). In addition, no significant differences were detected in the mitotic index or proliferative index at examined doses (up to 20 μm). In conclusion, NCTD is genotoxic to human cultured lymphocytes as measured by SCE assay.
去甲斑蝥素（NCTD）目前用于治疗多种癌症，如白血病、黑色素瘤和肝癌。NCTD的作用机制被认为涉及通过产生活性氧诱导癌细胞凋亡。在本研究中，使用姐妹染色单体交换（SCE）和染色体畸变（CA）试验研究了不同浓度（1、10和20μm）的NCTD对人淋巴细胞的遗传毒性作用。结果显示，NCTD以剂量依赖性方式显著提高了SCE率（p<0.05）。此外，NCTD显著增加了高频细胞的数量（SCE≥8，p<0.05）。然而，NCTD对CA率没有任何显著影响（p>0.05）。此外，在所检测的剂量（高达20μm）下，有丝分裂指数或增殖指数未检测到显著差异。总之，通过SCE试验测定，NCTD对人培养淋巴细胞具有遗传毒性。
