Lasker B A, Carle G F, Kobayashi G S, Medoff G
Department of Medicine, Washington University School of Medicine, St Louis, MO 63110.
Nucleic Acids Res. 1989 May 25;17(10):3783-93. doi: 10.1093/nar/17.10.3783.
Pulsed-field gel electrophoresis techniques were used to study chromosome-sized DNA molecules of C. albicans. Chromosome-sized DNA of two strains of Candida albicans has been resolved into 8 bands by orthogonal-field-alternation gel electrophoresis (OFAGE). Six bands were observed in chromosomal preparations of C. albicans using field-inversion gel electrophoresis (FIGE). Differences in the electrophoretic mobilities of bands of the strains of C. albicans examined suggests that chromosome-length polymorphisms exist and make it difficult to correlate the banding patterns among strains. These correlations were facilitated, however, by assignment of C. albicans chromosomes by hybridization using a collection of cloned DNA probes specific for each of the 8 observed bands. Southern blotting showed that the 6 FIGE bands consisted of 4 singlets and 2 comigrating doublets, accounting for the 8 bands observed by OFAGE analysis. The agreement between OFAGE and FIGE analysis suggests that the C. albicans haploid genome contains a minimum of 8 chromosomes.
脉冲场凝胶电泳技术被用于研究白色念珠菌的染色体大小的DNA分子。两株白色念珠菌的染色体大小的DNA已通过正交交变电场凝胶电泳（OFAGE）分离成8条带。使用场反转凝胶电泳（FIGE）在白色念珠菌的染色体制备物中观察到6条带。所检测的白色念珠菌菌株条带的电泳迁移率差异表明存在染色体长度多态性，并且难以使菌株间的条带模式相互关联。然而，通过使用针对观察到的8条带中的每条带的一组克隆DNA探针进行杂交来指定白色念珠菌染色体，促进了这些关联。Southern印迹显示，6条FIGE带由4个单条带和2个共迁移的双条带组成，这解释了OFAGE分析中观察到的8条带。OFAGE和FIGE分析之间的一致性表明白色念珠菌单倍体基因组至少包含8条染色体。
