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一维脉冲场凝胶电泳（ODPFGE）的制备、操作及脉冲策略

Preparation, manipulation, and pulse strategy for one-dimensional pulsed-field gel electrophoresis (ODPFGE).

作者信息

Noolandi J, Turmel C

机构信息

Xerox Research Centre of Canada, Mississauga, Ontario, Canada.

出版信息

Mol Biotechnol. 1995 Aug;4(1):25-43. doi: 10.1007/BF02907469.

DOI:10.1007/BF02907469
PMID:8521038
Abstract

The underlying principles for zero-integrated-field electrophoresis (ZIFE) pulses and more general forward-biased pulse schemes are reviewed for one-dimensional pulsed-field gel electrophoresis (ODPFGE) separations of large DNA molecules. Detailed descriptions of materials, preparation protocols, hardware requirements, and procedures are given. A variety of gel pictures for known yeast DNA markers are shown.

摘要

针对一维脉冲场凝胶电泳（ODPFGE）分离大DNA分子的情况，回顾了零积分场电泳（ZIFE）脉冲及更通用的正向偏置脉冲方案的基本原理。给出了材料、制备方案、硬件要求及操作步骤的详细描述。展示了多种已知酵母DNA标记的凝胶图片。

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本文引用的文献

1
Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).通过一维脉冲场凝胶电泳（ODPFGE）制备和分离脊椎动物的完整染色体
Methods Mol Biol. 1992;12:135-43. doi: 10.1385/0-89603-229-9:135.
2
Preparation, restriction, and hybridization analysis of Mammalian genomic DNA for pulsed-field gel electrophoresis.
Methods Mol Biol. 1992;12:107-28. doi: 10.1385/0-89603-229-9:107.
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
4
Development and use of metaphase chromosome flow-sorting methodology to obtain recombinant phage libraries enriched for parts of the human X chromosome.中期染色体流式分选方法的开发与应用，以获得富含人类X染色体部分区域的重组噬菌体文库。
Cytometry. 1984 Mar;5(2):101-7. doi: 10.1002/cyto.990050202.
5
Optimized conditions for pulsed field gel electrophoretic separations of DNA.用于DNA脉冲场凝胶电泳分离的优化条件。
Nucleic Acids Res. 1988 Aug 11;16(15):7563-82. doi: 10.1093/nar/16.15.7563.
6
Quantitative analysis of the three regimes of DNA electrophoresis in agarose gels.琼脂糖凝胶中DNA电泳三种状态的定量分析。
Biopolymers. 1988 Mar;27(3):509-24. doi: 10.1002/bip.360270311.
7
Pulsed-field electrophoresis: application of a computer model to the separation of large DNA molecules.脉冲场电泳：计算机模型在大DNA分子分离中的应用
Proc Natl Acad Sci U S A. 1987 Nov;84(22):8011-5. doi: 10.1073/pnas.84.22.8011.
8
Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome.粟酒裂殖酵母基因组的Not I限制酶切图谱构建
Nucleic Acids Res. 1989 Apr 11;17(7):2801-18. doi: 10.1093/nar/17.7.2801.
9
Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques.利用脉冲场凝胶电泳技术分离白色念珠菌染色体大小DNA的比较。
Nucleic Acids Res. 1989 May 25;17(10):3783-93. doi: 10.1093/nar/17.10.3783.
10
Molecular detrapping and band narrowing with high frequency modulation of pulsed field electrophoresis.脉冲场电泳高频调制下的分子解捕获与条带变窄
Nucleic Acids Res. 1990 Feb 11;18(3):569-75. doi: 10.1093/nar/18.3.569.