Magee B B, Koltin Y, Gorman J A, Magee P T
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108-1095.
Mol Cell Biol. 1988 Nov;8(11):4721-6. doi: 10.1128/mcb.8.11.4721-4726.1988.
By using orthogonal-field alternating gel electrophoresis (OFAGE), field-inversion gel electrophoresis (FIGE), and contour-clamped homogeneous field gel electrophoresis (CHEF), we have clearly resolved 11 chromosomal bands from various Candida albicans strains. OFAGE resolves the smaller chromosomes better, while FIGE, which under our conditions causes the chromosomes to run in the reverse order of OFAGE, is more effective in separating the larger chromosomes. CHEF separates all chromosomes under some conditions, but these conditions do not often resolve homologs. The strains examined are highly polymorphic for chromosome size. Fourteen cloned Candida genes, isolated on the basis of conferral of new properties to or complementation of auxotrophic deficiencies in Saccharomyces cerevisiae, and three sequences of unknown function have been hybridized to Southern transfers of CHEF, FIGE, and OFAGE gels. Four sets of resolvable bands have been shown to be homologous chromosomes. On the basis of these data, we suggest that C. albicans has seven chromosomes. Genes have been assigned to the seven chromosomes. Two chromosomes identified genetically have been located on the electrophoretic karyotype.
通过使用正交场交变凝胶电泳（OFAGE）、脉冲场凝胶电泳（FIGE）和轮廓夹恒定电场凝胶电泳（CHEF），我们已清晰分辨出多种白色念珠菌菌株的11条染色体条带。OFAGE能更好地分辨较小的染色体，而在我们的条件下，FIGE使染色体以与OFAGE相反的顺序移动，在分离较大染色体方面更有效。CHEF在某些条件下能分离所有染色体，但这些条件往往无法分辨同源染色体。所检测的菌株在染色体大小上具有高度多态性。基于赋予酿酒酵母新特性或互补营养缺陷型的能力而分离出的14个克隆的念珠菌基因，以及三个功能未知的序列，已与CHEF、FIGE和OFAGE凝胶的Southern转移杂交。已证明四组可分辨的条带是同源染色体。基于这些数据，我们认为白色念珠菌有七条染色体。基因已被定位到这七条染色体上。通过遗传学鉴定的两条染色体已定位到电泳核型上。
