通过拉曼光谱对间充质干细胞复制性衰老进行无标记评估。
Label-free assessment of replicative senescence in mesenchymal stem cells by Raman microspectroscopy.
作者信息
Bai Hua, Li Haiyu, Han Zhibo, Zhang Cheng, Zhao Junfa, Miao Changyun, Yan Shulin, Mao Aibin, Zhao Hui, Han Zhongchao
机构信息
College of Electronic and Information Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China ;
出版信息
Biomed Opt Express. 2015 Oct 21;6(11):4493-500. doi: 10.1364/BOE.6.004493. eCollection 2015 Nov 1.
Abstract
Here, Raman microspectroscopy was employed to assess replicative senescence of mesenchymal stem cells (MSC). A regular spectral change related to the cell senescence was found in the ratio of two peaks at 1157 cm(-1) and 1174 cm(-1), which are assigned to C-C, C-N stretching vibrations in proteins and C-H bending vibrations in tyrosine and phenylalanine, respectively. With the cell aging, the ratio I1157 / I1174 exhibited a monotonic decline and showed small standard deviations, so that it can statistically distinguish between cells having slight changes in terms of aging. We propose that I1157 / I1174 can act as a characteristic spectral signature for label-free assessment of MSC senescence.
摘要
在此，采用拉曼光谱显微镜评估间充质干细胞（MSC）的复制性衰老。在1157 cm(-1)和1174 cm(-1)处两个峰的比值中发现了与细胞衰老相关的规律性光谱变化，这两个峰分别对应于蛋白质中的C-C、C-N伸缩振动以及酪氨酸和苯丙氨酸中的C-H弯曲振动。随着细胞老化，I1157 / I1174比值呈单调下降且标准差较小，因此它能够在统计学上区分衰老程度有轻微变化的细胞。我们提出I1157 / I1174可作为无标记评估MSC衰老的特征光谱标志。
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