他莫昔芬对东莨菪碱诱导的去卵巢大鼠空间和非空间学习记忆障碍及脑组织氧化损伤的影响。

The effects of tamoxifen on spatial and nonspatial learning and memory impairments induced by scopolamine and the brain tissues oxidative damage in ovariectomized rats.

作者信息

Karimi Sareh, Hejazian Seyed Hassan, Alikhani Vajiheh, Hosseini Mahmoud

机构信息

Department of Physiology, Shahid Sadoghi University of Medical Sciences, Yazd, Iran.

Neurogenic Inflammation Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

出版信息

Adv Biomed Res. 2015 Sep 28;4:196. doi: 10.4103/2277-9175.166132. eCollection 2015.

BACKGROUND

Modulatory effects of tamoxifen (TAM) on the central nervous system have been reported. The effects of TAM on spatial and nonspatial learning and memory impairments induced by scopolamine and the brain tissues oxidative damage was investigated.

MATERIALS AND METHODS

The ovariectomized (OVX) rats were divided and treated: (1) Control (saline), (2) scopolamine (Sco; 2 mg/kg, 30 min before behavioral tests), (3-5) Sco-TAM 1, Sco-TAM 3 and Sco-TAM 10. TAM (1, 3 or 10 mg/kg; i.p.) was daily administered for 6 weeks.

RESULTS

In Morris water maze (MWM), both the latency and traveled distance in the Sco-group were higher than control (P < 0.001) while, in the Sco-TAM 10 group it was lower than Sco-group (P < 0.05). In passive avoidance test, the latency to enter the dark compartment was higher than control (P < 0.05 - P < 0.01). Pretreatment by all three doses of TAM prolonged the latency to enter the dark compartment compared to Sco-group (P < 0.05 - P < 0.001). The brain tissues malondialdehyde (MDA) concentration was increased while, superoxide dismutase activity (SOD) decreased in the Sco-group compared to control (P < 0.05 - P < 0.01). Pretreatment by TAM lowered the concentration of MDA while, increased SOD compared to Sco-group (P < 0.05 - P < 0.001).

CONCLUSIONS

It is suggested that TAM prevents spatial and nonspatial learning and memory impairments induced by scopolamine in OVX rats. The possible mechanism(s) might at least in part be due to protection against the brain tissues oxidative damage.

背景

已报道他莫昔芬（TAM）对中枢神经系统具有调节作用。研究了TAM对东莨菪碱诱导的空间和非空间学习记忆障碍以及脑组织氧化损伤的影响。

材料与方法

将去卵巢（OVX）大鼠分组并进行处理：（1）对照组（生理盐水），（2）东莨菪碱组（Sco；2mg/kg，行为测试前30分钟），（3 - 5）Sco - TAM 1组、Sco - TAM 3组和Sco - TAM 10组。TAM（1、3或10mg/kg；腹腔注射）每日给药，持续6周。

结果

在莫里斯水迷宫（MWM）中，Sco组的潜伏期和游动距离均高于对照组（P < 0.001），而Sco - TAM 10组低于Sco组（P < 0.05）。在被动回避试验中，进入暗室的潜伏期高于对照组（P < 0.05 - P < 0.01）。与Sco组相比，三种剂量的TAM预处理均延长了进入暗室的潜伏期（P < 0.05 - P < 0.001）。与对照组相比，Sco组脑组织丙二醛（MDA）浓度升高，超氧化物歧化酶活性（SOD）降低（P < 0.05 - P < 0.01）。与Sco组相比，TAM预处理降低了MDA浓度，增加了SOD（P < 0.05 - P < 0.001）。