Sun Lingmei, Wu Quli, Liao Kai, Yu Peihang, Cui Qiuhong, Rui Qi, Wang Dayong
Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School of Southeast University, Nanjing 210009, China.
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Chemosphere. 2016 Feb;144:2392-400. doi: 10.1016/j.chemosphere.2015.11.028. Epub 2015 Nov 22.
Contribution of chemical components in coal combustion related fine particulate matter (PM2.5) to its toxicity is largely unclear. We focused on heavy metals in PM2.5 to investigate their contribution to toxicity formation in Caenorhabditis elegans. Among 8 heavy metals examined (Fe, Zn, Pb, As, Cd, Cr, Cu, and Ni), Pb, Cr, and Cu potentially contributed to PM2.5 toxicity in wild-type nematodes. Combinational exposure to any two of these three heavy metals caused higher toxicity than exposure to Pb, Cr, or Cu alone. Toxicity from the combinational exposure to Pb, Cr, and Cu at the examined concentrations was higher than exposure to PM2.5 (100 mg/L). Moreover, mutation of sod-2 or sod-3 gene encoding Mn-SOD increased susceptibility in nematodes exposed to Fe, Zn, or Ni, although Fe, Zn, or Ni at the examined concentration did not lead to toxicity in wild-type nematodes. Our results highlight the potential contribution of heavy metals to PM2.5 toxicity in environmental organisms.
煤燃烧相关细颗粒物（PM2.5）中化学成分对其毒性的贡献在很大程度上尚不清楚。我们聚焦于PM2.5中的重金属，以研究它们对线虫毒性形成的贡献。在所检测的8种重金属（铁、锌、铅、砷、镉、铬、铜和镍）中，铅、铬和铜可能对线虫野生型的PM2.5毒性有贡献。这三种重金属中任意两种的组合暴露所导致的毒性高于单独暴露于铅、铬或铜。在所检测浓度下，铅、铬和铜的组合暴露毒性高于暴露于PM2.5（100毫克/升）。此外，编码锰超氧化物歧化酶的sod - 2或sod - 3基因突变增加了线虫对铁、锌或镍暴露的易感性，尽管在所检测浓度下铁、锌或镍不会导致线虫野生型产生毒性。我们的结果凸显了重金属对环境生物中PM2.5毒性的潜在贡献。
