Kim Eun-Hee, Kang Sun-Young, Park Bog-Im, Kim Young-Hoi, Lee Young-Rae, Hoe Jin-Hee, Choi Na-Young, Ra Ji-Young, An So-Youn, You Yong-Ouk
Department of Pediatric Dentistry, School of Dentistry, Wonkwang University, Iksan 54538, Republic of Korea.
Department of Oral Biochemistry, School of Dentistry, Wonkwang University, Iksan 54538, Republic of Korea.
Evid Based Complement Alternat Med. 2016;2016:2396404. doi: 10.1155/2016/2396404. Epub 2016 May 11.
Chamaecyparis obtusa (C. obtusa) is known to have antimicrobial effects and has been used as a medicinal plant and in forest bathing. This study aimed to evaluate the anticariogenic activity of essential oil of C. obtusa on Streptococcus mutans, which is one of the most important bacterial causes of dental caries and dental biofilm formation. Essential oil from C. obtusa was extracted, and its effect on bacterial growth, acid production, and biofilm formation was evaluated. C. obtusa essential oil exhibited concentration-dependent inhibition of bacterial growth over 0.025 mg/mL, with 99% inhibition at a concentration of 0.2 mg/mL. The bacterial biofilm formation and acid production were also significantly inhibited at the concentration greater than 0.025 mg/mL. The result of LIVE/DEAD® BacLight™ Bacterial Viability Kit showed a concentration-dependent bactericidal effect on S. mutans and almost all bacteria were dead over 0.8 mg/mL. Real-time PCR analysis showed that gene expression of some virulence factors such as brpA, gbpB, gtfC, and gtfD was also inhibited. In GC and GC-MS analysis, the major components were found to be α-terpinene (40.60%), bornyl acetate (12.45%), α-pinene (11.38%), β-pinene (7.22%), β-phellandrene (3.45%), and α-terpinolene (3.40%). These results show that C. obtusa essential oil has anticariogenic effect on S. mutans.
钝叶扁柏已知具有抗菌作用，已被用作药用植物并用于森林浴。本研究旨在评估钝叶扁柏精油对变形链球菌的防龋活性，变形链球菌是龋齿和牙菌斑形成的最重要细菌原因之一。提取了钝叶扁柏的精油，并评估了其对细菌生长、产酸和生物膜形成的影响。钝叶扁柏精油在浓度超过0.025mg/mL时表现出浓度依赖性的细菌生长抑制作用，在浓度为0.2mg/mL时抑制率达99%。在浓度大于0.025mg/mL时，细菌生物膜形成和产酸也受到显著抑制。LIVE/DEAD® BacLight™细菌活力试剂盒的结果显示对变形链球菌有浓度依赖性的杀菌作用，在浓度超过0.8mg/mL时几乎所有细菌都死亡。实时PCR分析表明，一些毒力因子如brpA、gbpB、gtfC和gtfD的基因表达也受到抑制。在气相色谱和气相色谱-质谱分析中，发现主要成分是α-松油烯（40.60%）、乙酸龙脑酯（12.45%）、α-蒎烯（11.38%）、β-蒎烯（7.22%）、β-水芹烯（3.45%）和α-松油烯（3.40%）。这些结果表明钝叶扁柏精油对变形链球菌具有防龋作用。
