Cazella Luciane Neris, Glamoclija Jasmina, Soković Marina, Gonçalves José Eduardo, Linde Giani Andrea, Colauto Nelson Barros, Gazim Zilda Cristiani
Graduate Program in Biotechnology Applied to Agriculture, Chemistry Laboratory of Natural Products, Paranaense University, Umuarama, Brazil.
Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
Front Plant Sci. 2019 Jan 29;10:27. doi: 10.3389/fpls.2019.00027. eCollection 2019.
DC (Asteraceae) is a Brazilian native bush tree, and its leaf essential oil has been reported to possess some biological activities, but the antimicrobial activity of its aerial part essential oil at the flowering period is unknown or little studied, mainly against agents that cause foodborne diseases. Thus, this study aimed to determine the chemical composition and evaluate the antimicrobial activity of the essential oil of aerial part at flowering period. This essential oil was obtained by hydro distillation and its chemical composition was determined by gas chromatography coupled with mass spectrometry (GC-MS). The minimum inhibitory concentration, minimum bactericidal concentration, and minimum fungicidal concentration of the essential oil were evaluated against eight bacteria and eight fungi using 96-well microtiter plates. The essential oil yield was 1.8 ± 0.07%, and spathulenol (27%) and -nerolidol (23%), both oxygenated sesquiterpenes, were the major compounds found among 30 chemical constituents identified. The essential oil presented bacteriostatic and bactericidal activities, mainly against , and , and also fungistatic and fungicidal activities. However, its antibacterial activity was more effective than the antifungal one by using the essential oil at lower concentrations. Essential oil of may be a potential alternative for food applications in order to reduce synthetic chemicals in a more sustainable food industry.
菊科植物DC是一种原产于巴西的灌木树,据报道其叶精油具有一些生物活性,但其花期地上部分精油的抗菌活性未知或研究较少,主要针对引起食源性疾病的病原体。因此,本研究旨在确定其花期地上部分精油的化学成分并评估其抗菌活性。该精油通过水蒸馏获得,其化学成分通过气相色谱-质谱联用(GC-MS)测定。使用96孔微量滴定板评估该精油对8种细菌和8种真菌的最低抑菌浓度、最低杀菌浓度和最低杀真菌浓度。精油得率为1.8±0.07%,在鉴定出的30种化学成分中,主要成分是spathulenol(27%)和β-橙花叔醇(23%),二者均为氧化倍半萜。该精油具有抑菌和杀菌活性,主要针对[具体细菌名称未给出]、[具体细菌名称未给出]和[具体细菌名称未给出],也具有抑真菌和杀真菌活性。然而,在较低浓度下使用该精油时,其抗菌活性比抗真菌活性更有效。菊科植物DC的精油可能是食品应用中的一种潜在替代品,以便在更可持续的食品工业中减少合成化学品的使用。