Wysok B, Uradziński J, Wojtacka J
Pol J Vet Sci. 2015;18(3):579-86. doi: 10.1515/pjvs-2015-0075.
The study was carried out to determine the cytotoxin production by Campylobacter spp. isolated from slaughtered cattle and swine in north-eastern Poland. In total three commercial slaughterhouses were sampled during one year. Carcass swabs were taken to detect the level of Campylobacter spp. contamination. Campylobacter spp. was found in 50 (34%) out of 147 swine carcasses examined. PCR analysis revealed 4 (8%) isolates to be C. jejuni, and 46 (92%) to be C. coli. From a total of 373 bovine carcasses, Campylobacter spp. were isolated from 49 (13.1%) samples. The results regarding the occurrence of cdt genes associated with cytotoxicity indicated that 100% of C. jejuni and 67.4% C. coli obtained from pigs had all three cdtA, cdtB and cdtC genes. In case of C. jejuni strains isolated from cattle all cdt genes were confirmed in 93.9% isolates. The isolates possessesing all cdt genes had higher cytotoxic activity against cell lines used. The isolates both from cattle and swine were characterized by the highest cytotoxicity against HeLa cells. The values obtained reached 80.8% for C. jejuni isolates from cattle and 76.2% for C. jejuni and 69.0% for C. coli isolates from swine. High prevalence of cytotoxicity in Campylobacter spp. indicates a significant epidemiological role of this pathogen in human infections.
开展该研究以确定从波兰东北部屠宰牛和猪中分离出的弯曲杆菌属细菌产生细胞毒素的情况。在一年时间里，总共对三家商业屠宰场进行了采样。采集胴体拭子以检测弯曲杆菌属细菌的污染水平。在147头检查的猪胴体中，有50头（34%）检测到弯曲杆菌属细菌。PCR分析显示，4株（8%）分离株为空肠弯曲菌，46株（92%）为大肠弯曲菌。在总共373头牛胴体中，从49份（13.1%）样本中分离出弯曲杆菌属细菌。关于与细胞毒性相关的cdt基因出现情况的结果表明，从猪中获得的空肠弯曲菌100%以及大肠弯曲菌67.4%具有全部三个cdtA、cdtB和cdtC基因。在从牛中分离出的空肠弯曲菌菌株中，93.9%的分离株所有cdt基因均得到确认。拥有所有cdt基因的分离株对所用细胞系具有更高的细胞毒性活性。来自牛和猪的分离株对HeLa细胞的细胞毒性最高。从牛中分离出的空肠弯曲菌分离株的值达到80.8%，从猪中分离出的空肠弯曲菌为76.2%，大肠弯曲菌为69.0%。弯曲杆菌属细菌中细胞毒性的高流行率表明该病原体在人类感染中具有重要的流行病学作用。
